Method of x-ray anomalous diffraction for lipid structures.

Biophysical Journal
Wangchen WangHuey W Huang

Abstract

The structures of the unit cells of lipid phases that exhibit long-range crystalline order but short-range liquid-like disorder are of biological interests. In particular, the recently discovered rhombohedral phase has a unit cell containing either the structure of a membrane fusion intermediate state or that of a peptide-induced transmembrane pore, depending on the lipid composition and participating peptides. Diffraction from such systems generally presents a difficult phase problem. The existing methods of phase determination all have their limitations. Therefore it is of general interest to develop a new phasing method. The method of multi-wavelength anomalous dispersion is routinely used in protein crystallography, but the same method is difficult for lipid systems for the practical reason that the commonly used lipid samples for diffraction do not have a well-defined thickness. Here we describe a practical approach to use the multi-wavelength anomalous dispersion method for lipid structures. The procedure is demonstrated with the lamellar phase of a brominated lipid. The method is general to all phases as long as anomalous diffraction is applicable.

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Dec 29, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Lai DingHuey W Huang

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Citations

Nov 7, 2008·Proceedings of the National Academy of Sciences of the United States of America·Shuo QianHuey W Huang
Aug 14, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ming-Tao LeeHuey W Huang
Jan 18, 2008·Biophysical Journal·Shuo QianHuey W Huang
Mar 21, 2007·Biophysical Journal·Wei-Chin HungHuey W Huang
Apr 8, 2016·Chemical Reviews·Tao LiByeongdu Lee

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