Method parameters' impact on mortality and variability in mouse stroke experiments: a meta-analysis

Scientific Reports
Edvin IngbergJakob O Ström

Abstract

Although hundreds of promising substances have been tested in clinical trials, thrombolysis currently remains the only specific pharmacological treatment for ischemic stroke. Poor quality, e.g. low statistical power, in the preclinical studies has been suggested to play an important role in these failures. Therefore, it would be attractive to use animal models optimized to minimize unnecessary mortality and outcome variability, or at least to be able to power studies more exactly by predicting variability and mortality given a certain experimental setup. The possible combinations of methodological parameters are innumerous, and an experimental comparison of them all is therefore not feasible. As an alternative approach, we extracted data from 334 experimental mouse stroke articles and, using a hypothesis-driven meta-analysis, investigated the method parameters' impact on infarct size variability and mortality. The use of Swiss and C57BL6 mice as well as permanent occlusion of the middle cerebral artery rendered the lowest variability of the infarct size while the emboli methods increased variability. The use of Swiss mice increased mortality. Our study offers guidance for researchers striving to optimize mouse stroke models.

References

Nov 4, 2000·Stroke; a Journal of Cerebral Circulation·A MajidC Y Hsu
Feb 13, 2001·BMJ : British Medical Journal·J A Sterne, G Davey Smith
Feb 3, 2006·Annals of Neurology·Victoria E O'CollinsDavid W Howells
Mar 10, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Ulrich Dirnagl
Apr 14, 2006·Neuroimmunomodulation·Olaf Schulte-HerbrüggenAndreas Meisel
Sep 27, 2007·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·J HuangE S Connolly
Feb 23, 2008·Cerebrovascular Diseases·Matthias EndresUlrich Dirnagl
Mar 29, 2008·Stroke; a Journal of Cerebral Circulation·Wenri ZhangNabil J Alkayed
Apr 3, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Hyung-Hwan KimJames K Liao
Apr 12, 2008·Stroke; a Journal of Cerebral Circulation·Xiaoying WangEng H Lo
May 2, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Eunhee KimSunghee Cho
May 3, 2008·Stroke; a Journal of Cerebral Circulation·Takashi MoriTerrence Town
May 13, 2008·Lancet·Geoffrey A DonnanStephen M Davis
May 20, 2008·Neurobiology of Disease·Ulkan KilicDirk M Hermann
Jun 19, 2008·The Journal of Experimental Medicine·David Varga-SzaboBernhard Nieswandt
Jun 19, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Motoaki FujimotoKazuhiko Nozaki
Jun 26, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Adám DénesKrisztina J Kovács
Jul 17, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Yun Seon SongPak H Chan
Jul 19, 2008·Stroke; a Journal of Cerebral Circulation·Malcolm R MacleodGeoffrey A Donnan
Jul 29, 2008·Stroke; a Journal of Cerebral Circulation·Sönke SchwartingHarald Neumann
Jul 29, 2008·Stroke; a Journal of Cerebral Circulation·Junji NakamuraKazuhiro Nagata
Jul 31, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Keita MayanagiDavid W Busija
Aug 16, 2008·Stroke; a Journal of Cerebral Circulation·Malcolm R MacleodDavid W Howells

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