Abstract
To precisely purify and study aged (senescent) cells, we have designed, fabricated, and demonstrated a novel diamond-structure (DS) microfluidic filter. Nonuniform flow velocities within the microfilter channel can compromise microfluidic filter performance, but with this new diamond structure, further optimized via simulation, we achieve a uniform microfilter flow field, improving the throughput of size-based separation of senescent cells, as obtained by 39-passaged human dermal fibroblasts. After separating these aged cells into two groups, consisting of large- and small-sized cells, we assessed senescence by measuring lipofuscin accumulation and β-galactosidase activity. Our results reveal that even though these senescent cells had been equivalently passaged in culture, a high degree of size distribution and senescent phenotype heterogeneity was observed. In particular, the smaller-sized cells tended to express a younger phenotype while the larger aged cells demonstrated an older phenotype. We suggest that size-based separation of senescent cells, subtyped into small- and large-sized cohorts, offers an alternative method to purify such aged cells, thereby enabling more precise study of the mechanisms of aging, autophagy impa...Continue Reading
References
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J R SmithE L Schneider
Jul 1, 1979·The Journal of Investigative Dermatology·E L Schneider
Jul 1, 1979·The Journal of Investigative Dermatology·L Hayflick
Sep 1, 1984·Experimental Cell Research·E Wang, D Gundersen
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·G P DimriO Pereira-Smith
Nov 1, 1993·Experimental Cell Research·W R ReenstraB A Gilchrest
Apr 9, 1998·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·A Terman, U T Brunk
Nov 20, 2002·Archives of Dermatology·Mina Yaar, Mark S Eller
Mar 1, 1965·Experimental Cell Research·L HAYFLICK
Jul 13, 2004·Annals of the New York Academy of Sciences·Alexei TermanUlf T Brunk
Dec 22, 2004·The Journal of Investigative Dermatology·Chi-Hyun ParkJin Ho Chung
Feb 28, 2009·Analytical Chemistry·Sungyoung ChoiJe-Kyun Park
May 8, 2009·Cellular and Molecular Life Sciences : CMLS·Eun Seong HwangHyun Tae Kang
May 11, 2010·PloS One·Minseok S KimJe-Kyun Park
Jan 18, 2011·Lab on a Chip·Laure SaiasJean-Louis Viovy
Feb 16, 2011·The Journal of Cell Biology·Francis Rodier, Judith Campisi
Aug 23, 2011·PloS One·Hyun Tae KangSang Chul Park
Jun 12, 2012·Lab on a Chip·Minseok S KimJae Chan Park
Feb 13, 2013·Small·Minseok S KimJeong-Gun Lee
Mar 20, 2014·Analytical Chemistry·Jong-Myeon ParkNam Huh
Sep 12, 2014·Analytical Chemistry·Ryo OyobikiNobuhide Doi
Oct 25, 2014·Analytical Chemistry·Yi-Hsuan SuNathan S Swami
Oct 29, 2014·Analytical Chemistry·Brian D Plouffe, Shashi K Murthy
Dec 3, 2014·Trends in Cell Biology·Ryan R Driskell, Fiona M Watt
Apr 8, 2015·Proceedings of the National Academy of Sciences of the United States of America·Peng LiTony Jun Huang
Citations
May 24, 2016·Ageing Research Reviews·Mantas MatjusaitisAlexandra Stolzing
May 4, 2017·Experimental Gerontology·Jia ChenYa-Ni He
Jun 2, 2020·Biomicrofluidics·Zhengkun ChenChunyi Wen
Jun 21, 2019·Biotechnology Journal·Joshur AngSteve Oh
Oct 28, 2019·PloS One·Seung Joon LeeMinseok S Kim