Methodical problems for evidence of the hepatitis-B-surface and hepatitis-B-core antigens in tissue (author's transl)

Acta histochemica
G Hess, W Arnold

Abstract

Liver biopsy material of 22 in the serum HBsAg positive patients was tested with the fluorescent antibody technique for the localization of HBcAg and HBsAg in the liver tissue. Comparative studies were done with the following tissue preparation techniques: Cryostat technique, freeze drying, freeze substitution, cold ethanol paraffin embedding technique (SAINTE MARIE) and isolated liver cells. The investigations revealed the following results: 1. No HB-components could be detected with the cold ethanol paraffin embedding technique and freeze substitution. 2. Using the cryostat technique HBsAg could be demonstrated in 16/22 (cytoplasmatic localization) and HBcAg in 8/22 (nuclear localization). 3. With freeze drying HBsAg and HBcAg could be found in the same cases. The excellent tissue preparation allowed a correct localization of the HB-components to the cell structure. 4. In comparison to cryostat sections in isolated liver cells HBcAg could be demonstrated in 11/16 and HBcAg in 8/8 cases.

References

Jan 1, 1975·Journal of Immunological Methods·M B Ray, V J Desmet
Oct 1, 1971·The Journal of Experimental Medicine·T S Edgington, D J Ritt
May 6, 1972·Lancet·S J HadziyannisA Afroudakis
Dec 1, 1972·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W Arnold, H von Mayersbach
Jan 19, 1973·Deutsche medizinische Wochenschrift·R MüllerH Deicher
Oct 20, 1973·Lancet·J H HoofnagleL F Barker
Dec 1, 1972·Journal of Clinical Pathology·T AkeyamaH Abe
Oct 1, 1972·The Journal of Infectious Diseases·K KrawczyńskiA Nowoslawski
Apr 1, 1973·The Journal of Infectious Diseases·W J BrzoskoA Nowoslawski
Jun 13, 1974·The New England Journal of Medicine·S KrugmanJ L Gerin
Jun 13, 1974·The New England Journal of Medicine·J H HoofnagleL F Barker
Feb 1, 1970·The Journal of Experimental Medicine·V E CoyneB S Blumberg
Jan 1, 1970·Klinische Wochenschrift·F PesendorferF Wewalka

Related Concepts

Immunofluorescence Assay
Hepatitis B Core Antigen
Hepatitis B Surface Antigens
Histological Techniques
Liver

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.