Methodologic research on TIMP-1, TIMP-2 detection as a new diagnostic index for hepatic fibrosis and its significance

World Journal of Gastroenterology : WJG
Qing-He NieYi-Zhan Cao

Abstract

To set up a new method to detect tissue inhibitors of metalloproteinase-1 and -2(TIMP-1 and TIMP-2) in sera of patients with hepatic cirrhosis, and to investigate the expression and location of TIMP-1 and TIMP-2 in liver tissue of patients with hepatic cirrhosis, and the correlation between TIMPs in liver and those in sera so as to discuss whether TIMPs can be used as a diagnosis index of hepatic fibrosis. The monoclonal antibodies (McAbs) of TIMP-1 and TIMP-2 were used to sensitize erythrocytes, and solid-phase absorption to sensitized erythrocytes (SPASE) was used to detect TIMP-1 and TIMP-2 in the sera of patients with hepatic cirrhosis. Meanwhile, with the method of in situ hybridization and immunohistochemistry, we studied the mRNA expression and antigen location of TIMP-1 and TIMP-2 in the livers of 40 hepatic cirrhosis patients with pathologic diagnosis. With SPASE, they were 16.4% higher in the acute hepatitis group, 33.3% higher in the chronic hepatitis group, and the positive rates were 73.6% and 61.2% respectively in sera of hepatic cirrhosis patients, which were remarkably higher than those in chronic hepatitis and acute hepatitis group (P<0.001). In 40 samples of hepatic cirrhosis tissues, all of them showed positi...Continue Reading

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Citations

Mar 13, 2003·World Journal of Gastroenterology : WJG·Xiao-Zhong WangBin Li
Dec 27, 2003·World Journal of Gastroenterology : WJG·Qing-He NieBo-Rong Pan
Mar 31, 2004·World Journal of Gastroenterology : WJG·Yan-Jun LuoLi Wang
Sep 10, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mallika KaviratneThomas A Wynn
Jan 1, 2003·World Journal of Gastroenterology : WJG·Hong-Lei Han, Zhen-Wei Lang
Dec 12, 2003·World Journal of Gastroenterology : WJG·Alicja Wiercinska-DrapaloDanuta Prokopowicz
May 24, 2006·World Journal of Gastroenterology : WJG·Qing-He NieYong-Xing Zhou

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