PMID: 6170207Apr 1, 1981Paper

Methodological approaches to antigen identification in soluble immune complexes; A model study

Acta Pathologica Et Microbiologica Scandinavica. Section C, Immunology
S HusbyP O Schiøtz

Abstract

The usefulness of different techniques for identification of immune complex-(IC) bound antigen(s) was investigated in two Ab-Ag model systems. The following approaches were used: 1) determination of IC-activity before and after removal of IC by adsorption to antibody-specific solid phase systems. 2) analysis of PEG-insoluble IC by reference line-rocket immunoelectrophoresis and 3) reprecipitation of PEG-insoluble IC in the presence of 125I-labelled antibody to suspected antigens and subsequent analysis of the distribution of the radioactivity. This latter approach, the use of a double PEG precipitation immunoradiometric assay, was found most useful and simple for identification of antigen(s) contained in soluble IC. The technique is most sensitive in demonstrating IC-bound antigen(s) when the IC are formed in slight to moderate antigen excess.

References

Jan 1, 1979·Journal of Immunological Methods·W Pernice, H H Sedlacek
Jan 1, 1979·Advances in Immunology·A N Theofilopoulos, F J Dixon
Mar 1, 1979·Molecular Immunology·D Male, I M Roitt
Jan 1, 1973·Scandinavian Journal of Immunology. Supplement·N Harboe, A Ingild

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