PMID: 2114053Mar 1, 1990Paper

Methodological approaches to evaluation of prostaglandin synthesis by isolated human thrombocytes

Voprosy medit︠s︡inskoĭ khimii
N G Geling

Abstract

Individual steps of prostaglandin-synthetase activity evaluation in human platelets are considered in detail, involving isolation of platelets, incubation of washed cells with labelled arachidonic or dihomo-gamma-linolenic acids, extraction of prostaglandins from incubation medium and their separation as well as quantitative estimation of the prostaglandins developed (nmoles/10(9) platelets/10 min). The methodical approaches suggested enabled not only to avoid some errors during estimation of the prostaglandin-synthetase activity in individual platelets but also to increase accuracy, sensitivity and specificity of the procedure as compared with similar methods.

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