PMID: 9428597Jan 15, 1998Paper

Methodological aspects for in vitro characterization of receptor binding using 11C-labeled receptor ligands: a detailed study with the benzodiazepine receptor antagonist [11C]Ro 15-1788

Nuclear Medicine and Biology
W SihverB Långström

Abstract

As a complement to in vivo studies with positron emission tomography (PET), it is desirable to perform in vitro characterization of newly developed 11C tracers. In this report we describe the technique for determination of receptor-ligand kinetics utilizing ligands labeled with the short-lived radionuclide 11C. The limitations and advantages are discussed. The benzodiazepine antagonist [11C]Ro 15-1788 was used as a model substance, and the use of storage phosphor plates for quantification of radioactivity was validated. Storage phosphor plates showed an excellent linear range (approximately 10[3]) and acceptable resolution (approximately 0.5 mm). Receptor-ligand kinetics, including depletion, association and dissociation, saturation and displacement were evaluated with good results through the use of short-lived radiotracers and storage phosphor plates.

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Citations

Feb 24, 2007·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Bengt LångströmHåkan Hall
Apr 18, 2000·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·F WuB Långström
Oct 21, 2015·Expert Opinion on Drug Discovery·Wilhelmus E A de WitteElizabeth C M de Lange
May 9, 2009·Nuclear Medicine and Biology·Maria ErlandssonBengt Långström
Jun 14, 2005·British Journal of Pharmacology·Joost BartN Harry Hendrikse
Jan 1, 2008·Journal of Neuroscience Methods·Remco J J KnolJan Booij
Jul 5, 2007·British Journal of Cancer·J BartN H Hendrikse
Apr 11, 2019·International Psychogeriatrics·Alys W GriffithsClaire A Surr
Jun 1, 1999·Journal of Neurochemistry·Y WatanabeY Watanabe
Dec 29, 2020·European Journal of Nuclear Medicine and Molecular Imaging·Laura KueblerKristina Herfert
Nov 27, 2018·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·Mingzhang GaoQi-Huang Zheng
Apr 10, 2018·Bioorganic & Medicinal Chemistry Letters·Mingzhang GaoQi-Huang Zheng
Dec 2, 2004·Analytical Chemistry·Martin LavénBengt Långström

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