Methodological Guidelines for Accurate Detection of Viruses in Wild Plant Species

Applied and Environmental Microbiology
Christelle LacroixCarolyn M Malmstrom

Abstract

Ecological understanding of disease risk, emergence, and dynamics and of the efficacy of control strategies relies heavily on efficient tools for microorganism identification and characterization. Misdetection, such as the misclassification of infected hosts as healthy, can strongly bias estimates of disease prevalence and lead to inaccurate conclusions. In natural plant ecosystems, interest in assessing microbial dynamics is increasing exponentially, but guidelines for detection of microorganisms in wild plants remain limited, particularly so for plant viruses. To address this gap, we explored issues and solutions associated with virus detection by serological and molecular methods in noncrop plant species as applied to the globally important Barley yellow dwarf virus PAV (Luteoviridae), which infects wild native plants as well as crops. With enzyme-linked immunosorbent assays (ELISA), we demonstrate how virus detection in a perennial wild plant species may be much greater in stems than in leaves, although leaves are most commonly sampled, and may also vary among tillers within an individual, thereby highlighting the importance of designing effective sampling strategies. With reverse transcription-PCR (RT-PCR), we demonstrate ...Continue Reading

References

Jan 1, 1986·Cold Spring Harbor Symposia on Quantitative Biology·K MullisH Erlich
Apr 1, 1995·PCR Methods and Applications·K H Roux
Jul 11, 1995·Nucleic Acids Research·S H De BoerS Chittaranjan
Oct 1, 1995·Journal of Clinical Microbiology·D L WiedbraukA M Drevon
Mar 1, 1996·Applied and Environmental Microbiology·C A Kreader
Mar 1, 1997·Journal of Medical Microbiology·M Vaneechoutte, J Van Eldere
Nov 5, 1997·Applied and Environmental Microbiology·I G Wilson
Dec 2, 2000·Journal of Clinical Microbiology·W Abu Al-Soud, P Rådström
Dec 25, 2002·Emerging Infectious Diseases·Daniel T HaydonM Karen Laurenson
May 6, 2003·Annual Review of Phytopathology·Stewart Gray, Frederick E Gildow
Sep 19, 2003·International Microbiology : the Official Journal of the Spanish Society for Microbiology·María M LópezMariano Cambra
Feb 7, 2004·Molecular Biotechnology·Peter RådströmCharlotta Löfström
Jan 1, 1997·Annual Review of Phytopathology·W A Miller, L Rasochová
Jul 6, 2004·Journal of Virological Methods·Carolyn M Malmstrom, Ruijie Shu
Dec 22, 2005·Journal of Agricultural and Food Chemistry·Donna S SmithSolke H De Boer
Feb 2, 2006·Oecologia·Lynn B MartinMartin Wikelski
Feb 21, 2006·Emerging Infectious Diseases·Mark E J Woolhouse, Sonya Gowtage-Sequeria
Feb 16, 2007·Evolution; International Journal of Organic Evolution·Martin R MillerMichael Boots
Mar 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth T BorerAndrew P Dobson
Jan 16, 2008·The Journal of Animal Ecology·K A LeeK C Klasing
Jul 12, 2008·Phytochemical Analysis : PCA·Giorgio GambinoIvana Gribaudo
Aug 6, 2008·Annual Review of Phytopathology·Eva H Stukenbrock, Bruce A McDonald
Dec 17, 2008·Environmental Health : a Global Access Science Source·Jill R StewartA Fredrick Holland
Jul 8, 2009·Pakistan Journal of Biological Sciences : PJBS·MirDavood OmraniDavood Agaverdizadae
Dec 18, 2009·Journal of Forensic Sciences·Kerry L OpelBruce R McCord
Feb 12, 2010·Cold Spring Harbor Protocols·Kenneth H Roux
Jul 14, 2010·Ecology Letters·James Patrick CroninCharles E Mitchell
Aug 1, 1996·Trends in Ecology & Evolution·B C Sheldon, S Verhulst
Jan 1, 2008·CSH Protocols·Peter RådströmPetra Wolffs
May 4, 2011·The Plant Journal : for Cell and Molecular Biology·In Sun HwangByung Kook Hwang
May 31, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ehsan KarimiSahida Ahmad
Sep 16, 2011·Forensic Science International. Genetics·Reza Alaeddini

❮ Previous
Next ❯

Citations

May 20, 2017·Annual Review of Phytopathology·Coralie PicardGaël Thébaud
Mar 27, 2021·Frontiers in Microbiology·Cleo AnastassopoulouYiannis Manoussopoulos

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.