PMID: 16643807Apr 29, 2006Paper

Methodological study on magnetic enzymic immunoassay for detecting free hCGbeta subunit

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Zhen-shi LiuXiangliang Yang

Abstract

To establish a novel magnetic enzyme immunoassay (MEIA) for detecting human choriogonadotropin free beta subunit (hCGbeta). Two monoclonal antibodies (mAb) were used for conjugating with FITC and with alkaline phosphatase(AP) respectively, which incorporated magnetic solid phase separation. Magnetic beads were coupled with sheep anti-FITC antibody as solid phase, and phenolphthalein monophosphate was used as substrate to set up MEIA for detecting hCGbeta. The sensitivity of hCGbeta MEIA kit reached 0.1 IU/L. The intraassay variation and inter-assay variation was 8.5% and 14% respectively, with the average recovery rate of dilution of 92.5%. The kit showed no cross reactivity to LH, TSH and FSH, while a cross reactivity of 1.2% to intact hCG at concentration of 1000 IU/L. The time of efficacy of hCGbeta MEIA kit was longer than 14 months. hCGbeta MEIA kit is better than hCGbeta radio immunoassay and ELISA kit, which can provide a high-qualitative and cheap hCGbeta kit for market.

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