Methodologies and perspectives of proteomics applied to filamentous fungi: from sample preparation to secretome analysis

International Journal of Molecular Sciences
Linda Bianco, Gaetano Perrotta

Abstract

Filamentous fungi possess the extraordinary ability to digest complex biomasses and mineralize numerous xenobiotics, as consequence of their aptitude to sensing the environment and regulating their intra and extra cellular proteins, producing drastic changes in proteome and secretome composition. Recent advancement in proteomic technologies offers an exciting opportunity to reveal the fluctuations of fungal proteins and enzymes, responsible for their metabolic adaptation to a large variety of environmental conditions. Here, an overview of the most commonly used proteomic strategies will be provided; this paper will range from sample preparation to gel-free and gel-based proteomics, discussing pros and cons of each mentioned state-of-the-art technique. The main focus will be kept on filamentous fungi. Due to the biotechnological relevance of lignocellulose degrading fungi, special attention will be finally given to their extracellular proteome, or secretome. Secreted proteins and enzymes will be discussed in relation to their involvement in bio-based processes, such as biomass deconstruction and mycoremediation.

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Jul 20, 2019·Current Protein & Peptide Science·Maria Priscila F LacerdaAna Marisa Fusco-Almeida
Feb 20, 2016·Electrophoresis·Jameel R Al-Obaidi
Mar 28, 2016·The Protein Journal·Jameel R Al-ObaidiAbu Seman Idris
Oct 23, 2020·Insects·Pamella Mingotti DiasPaulo Eduardo Teodoro
Dec 29, 2020·PeerJ·Nurlizah Abu BakarSiti Aisyah Alias
Feb 5, 2021·Journal of Proteomics·Murillo Peterlini TavaresValéria Monteze Guimarães

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Methods Mentioned

BETA
glycosylation
electrophoresis
chips

Software Mentioned

Mascot Science
Mascot
SILAC
MudPIT

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