Methodology for demonstrating and measuring the photocytotoxicity of fluoranthene to fish cells in culture

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Kristin SchirmerNiels C Bols

Abstract

Methodology was developed for quantifying the photocytotoxicity of fluoranthene to a gill cell line from rainbow trout for future use in screening polycyclic aromatic hydrocarbons for their relative photocytotoxicity to fish. Solubilization in a modified culture medium was achieved with and without foetal bovine serum (FBS) and with and without dimethyl sulfoxide (DMSO). FBS caused most of the fluoranthene to remain in solution and blocked photocytotoxicity if present during UV irradiation. DMSO had little effect on fluoranthene distribution in cell cultures but caused cells to be slightly more sensitive to the phototoxicity of fluoranthene. The indicator dyes alamar Blue() and 5-carboxyfluorescein diacetate acetoxymethyl ester were used to quantify cytotoxicity in two different ways-singly in two separate assays, and mixed together in a novel single assay, which saved time and material. With UV irradiation for 2 hr at a photon fluence rate of either 1.4 mumol UV-B/m(2)/sec (UV-A:UV-B, 1.5) or 1.1 mumol UV-B/m(2)/sec (UV-A:UV-B, 9.7), both dyes indicated increasing loss of viability with increasing doses of fluoranthene. EC(50) values ranged from 18 to 44 ng/ml (89-217 nM), with the alamar Blue assay being slightly more sensitive.

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Citations

Sep 2, 2000·Journal of Biochemical and Molecular Toxicology·K SchirmerN C Bols
Dec 25, 2012·In Vitro Cellular & Developmental Biology. Animal·Bryan SansomLucy E J Lee
Apr 5, 2005·Toxicon : Official Journal of the International Society on Toxinology·Ivanka TenevaKristin Schirmer
Oct 17, 2001·Developmental and Comparative Immunology·N C BolsL E Lee
Jun 28, 2003·Ophthalmic & Physiological Optics : the Journal of the British College of Ophthalmic Opticians (Optometrists)·Olanrewaju M Oriowo
Jul 14, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·David C VolzDaniel Villeneuve
Nov 2, 2013·Biologicals : Journal of the International Association of Biological Standardization·C VenkatesanG Sridevi
May 15, 2009·Environmental Health Perspectives·Susanne BastianKristin Schirmer
Sep 5, 2014·Archives of Environmental Contamination and Toxicology·Concepción García-GómezMar Babin
May 20, 2014·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Sarah J GignacLucy E J Lee
Mar 26, 2013·The Science of the Total Environment·Dolores FernándezMar Babín
Apr 4, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Linda M BrennanWilliam H van der Schalie
Aug 5, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Nynke I KramerKristin Schirmer
Mar 28, 2009·Chemosphere·Jung-Hwan KwonBeate I Escher
Sep 13, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Nynke I KramerEmanuela Testai
Sep 20, 2015·Toxicon : Official Journal of the International Society on Toxinology·Dalia PonceJuan José Dorantes-Aranda
Feb 17, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Vivian R DayehNiels C Bols
Oct 7, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Hyun-Yi YounJacob G Sivak
Jul 28, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·S J DeWitte-Orr, N C Bols
Sep 29, 2009·Environmental Pollution·Heike HildebrandKristin Schirmer

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