Methodology for optimally sized centrifugal partition chromatography columns

Journal of Chromatography. a
Sébastien CholletAlain Foucault

Abstract

Centrifugal Partition Chromatography (CPC) is a separation process based on the partitioning of solutes between two partially miscible liquid phases. There is no solid support for the stationary phase. The centrifugal acceleration is responsible for both stationary phase retention and mobile phase dispersion. CPC is thus a process based on liquid-liquid mass transfer. The separation efficiency is mainly influenced by the hydrodynamics of the phases in each cell of the column. Thanks to a visualization system, called "Visual CPC", it was observed that the mobile phase can flow through the stationary phase as a sheet, or a spray. Hydrodynamics, which directly governs the instrument efficiency, is directly affected during scale changes, and non-linear phenomena prevent the successful achievement of mastered geometrical scale changes. In this work, a methodology for CPC column sizing is proposed, based on the characterization of the efficiency of advanced cell shapes, taking into account the hydrodynamics. Knowledge about relationship between stationary phase volume, cell efficiency and separation resolution in CPC allowed calculating the optimum cell number for laboratory and industrial scale CPC application. The methodology is hi...Continue Reading

References

Nov 8, 1996·Journal of Chromatography. a·Y Ito, Y Ma
Apr 2, 2008·Journal of Chromatography. a·I A SutherlandO Intes
Dec 15, 2010·Journal of Chromatography. a·Elisabeth HopmannMirjana Minceva
Feb 7, 2012·Journal of Chromatography. a·Elisabeth Hopmann, Mirjana Minceva

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Citations

Jul 27, 2015·Journal of Chromatography. a·Elodie BoujuKarine Faure
Aug 6, 2016·Journal of Chromatography. a·Artak E Kostanyan, Andrey A Erastov
Nov 9, 2016·Journal of Chromatography. a·Norbert FumatKarine Faure
Feb 1, 2017·Journal of Separation Science·Małgorzata BojczukPaweł Hodurek
Dec 20, 2018·Journal of Phycology·Maria OrfanoudakiMarkus Ganzera
Apr 14, 2021·Annual Review of Chemical and Biomolecular Engineering·Raena Morley, Mirjana Minceva

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