Methodology for Sputum Induction and Laboratory Processing

Journal of Visualized Experiments : JoVE
Julien GuiotCatherine Moermans

Abstract

The technique of sputum induction and processing is a recognized non-invasive method allowing the collection and analysis of cells from the airways, which is interesting in various respiratory diseases like asthma, chronic obstructive pulmonary disease (COPD), chronic cough, or idiopathic pulmonary fibrosis. This technique is well tolerated, safe and non-invasive, but is currently limited to research services and specialized centers in clinical practice because it is technically demanding, time-consuming, and requires trained staff. The success rate of sputum induction and analysis is about 80%. Here, we describe the induction and laboratory processing of sputum samples. Sputum is induced by inhalation of hypertonic or isotonic saline with salbutamol. For the processing, we use the whole sputum technique. Dithiothreitol (DTT) is used to allow mucolysis of sputum samples. The primary aim of sputum processing is to obtain a differential cell count to study the cell types present in the airway lumen. Additional analyses may also be performed on sputum supernatant and sputum cells, which may allow further investigation into inflammatory processes and immune mechanisms. Examples include studying mediators in sputum supernatant and p...Continue Reading

Citations

Jul 25, 2019·Clinical Pharmacology and Therapeutics·Aernout van HaarstSabina Paglialunga
Jan 18, 2020·The European Respiratory Journal·Andrew Menzies-GowChristopher E Brightling
Dec 14, 2018·Frontiers in Microbiology·Christopher VinnardKeith Hamilton
Sep 1, 2019·Journal of Clinical Medicine·Julien GuiotMakon-Sébastien Njock
Aug 28, 2020·Frontiers in Microbiology·Hai-Yue LiuJin Su
Jan 13, 2021·International Journal of Molecular Sciences·Laura PastorDavid Sanz-Rubio

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