Methodology for the determination of bioavailability of labeled residues

Journal of Toxicology and Environmental Health
H E Gallo-Torres


Methods are described for the determination of bioavailability of lipo- and hydro- soluble compounds in the rat. These procedures involve catheterization of the portal vein and/or intestinal lymphatics to study absorption in unanesthetized animals. Catheterization of the common bile duct prevents recycling of materials through the entero-porto-hepato-biliary circulation (EPHBC). Steady-state conditions are ensured by constant infusion of bile or a solution of bile acids into the stomach or duodenum. The techniques and physiological considerations discussed in detail here have resulted in new proposed animal preparations, of value in the accurate determination of the bioavailability of labeled residues ingested by the second species. Since intake must be adequate to permit meaningful conclusions, the concentration of radioactivity in the harvested homogenized tissue is increased by lyophilization. The lyophilized material is compressed into pellets of adequate size. The animals, kept in restraining cages to prevent coprophagy, are allowed to eat the labeled residue spontaneously, if necessary, for 48 and even 72 hr. Bile, urine, and feces are collected for a sufficient length of time to allow quantitative excretion of the labele...Continue Reading


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