Methods development in multimodal chromatography with mobile phase modifiers using the steric mass action model

Journal of Chromatography. a
Hanne Sophie KarkovSteven M Cramer

Abstract

The ability to predict downstream protein purification processes is of great value in the biopharmaceutical industry; saving time, cost and resources. While many complex models exist, the appropriate use of simple models can be a useful tool for rapidly designing and optimizing processes as well as for risk analysis and establishing parameter ranges. In this study, the steric mass action isotherm is success-fully employed to predict the chromatographic behavior of a multimodal anionic Capto adhere systemin the presence of various mobile phase modifiers. An experimental protocol consisting of only a few column experiments is shown to be sufficient to establish the model. Proof of concept is carried out using human insulin and bovine serum albumin which have varying degrees of hydrophobicity, charge and size. Finally, the model predictions are verified under various experimental conditions and the unique selectivity of this multimodal system is explored and compared with traditional anion exchange resins.The simple model approach described here represents a rapid and useful method for model based process development of multimodal chromatography.

References

Oct 11, 2005·Journal of Chromatography. a·Niklas JakobssonBernt Nilsson
Apr 6, 2007·Protein Expression and Purification·Tsutomu ArakawaDaisuke Ejima
Feb 16, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·D Brynn Hibbert

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Citations

May 8, 2015·Biotechnology and Bioengineering·Hanne Sophie KarkovSteven M Cramer
Aug 1, 2015·Protein Expression and Purification·Atsushi HiranoTomoshi Kameda
Oct 1, 2015·Journal of Chromatography. a·William R KellerSteven M Cramer
Oct 27, 2017·Current Protein & Peptide Science·Atsushi HiranoTomoshi Kameda
Jun 10, 2020·Annual Review of Chemical and Biomolecular Engineering·Vijesh Kumar, Abraham M Lenhoff
Jul 16, 2017·Biotechnology and Bioengineering·Mo JiangRichard D Braatz
Nov 18, 2019·Journal of Chromatography. a·Leandro J BenediniFelipe F Furlan
Nov 28, 2017·Journal of Chromatography. a·Oliver PerssonBernt Nilsson
Jul 2, 2021·The Analyst·Sagar DhamaneRichard C Willson
Oct 30, 2021·Biotechnology and Bioengineering·Nicholas VecchiarelloSteven Cramer

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