Methods for Detecting N-Acyl Homoserine Lactone Production in Acinetobacter baumannii

Methods in Molecular Biology
Aimee R Paulk Tierney, Philip N Rather

Abstract

Acinetobacter baumannii cell-cell signaling is mediated by production and release of N-acyl homoserine lactones (AHLs), primarily N-(3-hydroxydodecanoyl)-L-homoserine lactone (3-hydroxy-C12-HSL). The secretion of this signal can be readily assessed via simple plate-based or thin-layer chromatography-based assays utilizing an Agrobacterium tumefaciens traG-lacZ containing biosensor. In this chapter, we describe methods to assay for secreted N-acyl homoserine lactone production in Acinetobacter baumannii.

Citations

Jun 30, 2021·Scientific Reports·Marisel Romina TuttobeneMaría Alejandra Mussi
Nov 23, 2021·Frontiers in Cellular and Infection Microbiology·Aimee R P TierneyPhilip N Rather

❮ Previous
Next ❯

Related Concepts

Related Feeds

Acinetobacter Infections

Acinetobacter infections have become common in hospitalized patients, especially in the intensive care unit setting and are difficult to treat due to their propensity to develop antimicrobial drug resistance. Discover the latest research on Acinetobacter Infections here.

© 2022 Meta ULC. All rights reserved