Methods for Determining the Cellular Functions of Vimentin Intermediate Filaments

Methods in Enzymology
Karen M RidgeRobert D Goldman

Abstract

The type III intermediate filament protein vimentin was once thought to function mainly as a static structural protein in the cytoskeleton of cells of mesenchymal origin. Now, however, vimentin is known to form a dynamic, flexible network that plays an important role in a number of signaling pathways. Here, we describe various methods that have been developed to investigate the cellular functions of the vimentin protein and intermediate filament network, including chemical disruption, photoactivation and photoconversion, biolayer interferometry, soluble bead binding assay, three-dimensional substrate experiments, collagen gel contraction, optical-tweezer active microrheology, and force spectrum microscopy. Using these techniques, the contributions of vimentin to essential cellular processes can be probed in ever further detail.

Citations

Jun 29, 2018·American Journal of Physiology. Lung Cellular and Molecular Physiology·Katharina HeinzelmannOliver Eickelberg
Dec 15, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Elena Stavenschi, David A Hoey
Sep 14, 2019·Journal of Oral and Maxillofacial Pathology : JOMFP·R Shesha PrasadAnisha Yaji
Jul 8, 2020·International Journal of Molecular Sciences·Irene RamosDolores Pérez-Sala
Jan 8, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Elham JavedEttickan Boopathi
Feb 8, 2021·European Journal of Medicinal Chemistry·Lei ZhangRuihuan Chen
Mar 7, 2021·International Journal of Molecular Sciences·Mohammed H AbuwarwarAnne L Fletcher

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