Methods for Establishment and Maintenance of Germ-Free Rat Models

Frontiers in Microbiology
Lingling QvLanjuan Li

Abstract

Numerous studies have demonstrated that the gut microbiota plays a vital role in human health and disease development. Although the number of studies on host-microbiota interactions have increased in recent years, the underlying pathogenesis of dysbiosis-related diseases are still largely unknown. Germ-free (GF) rodent models, with the animals housed in sterile isolators and completely free of microbiota, are useful tools to advance our understanding of host-microbiota relationship in vivo. Although protocols concerning the establishment and maintenance of GF mouse models have previously been reported, the establishment, maintenance and monitoring of GF rodents are labor-intensive, tedious and take experience and skills. The aim of our study was to establish a GF rat model for the following microbiota-related researches and provide an easy-to-use protocol for the establishment and maintenance of GF rat model in detail, including steps to set up the isolator, sterilize the flexible isolator bubble, import food, water and other supplies, and methods to acquire newborn GF rats, hand rearing of suckling GF rats and reproduction of GF offspring. During the hand feeding period, the body weight of suckling GF rats was weighed once a d...Continue Reading

References

Jan 1, 1981·Annual Review of Nutrition·B S Wostmann
Mar 1, 1996·Journal of Clinical Microbiology·D DymockW G Wade
Oct 10, 1955·The Journal of Nutrition·T D LUCKEYJ A REYNIERS
May 8, 1959·Annals of the New York Academy of Sciences·B E GUSTAFSSON
May 8, 1959·Annals of the New York Academy of Sciences·B E GUSTAFSSON
May 8, 1959·Annals of the New York Academy of Sciences·J R PLEASANTS
Jul 5, 2006·EMBO Reports·Ann M O'Hara, Fergus Shanahan
Nov 23, 2006·Seminars in Immunology·Karen SmithAndrew J Macpherson
Jan 1, 1946·Acta Anatomica·B GUSTAFSSON
Sep 3, 2013·Fertility and Sterility·Dongsheng HouChen Xu
Jul 1, 1992·World Journal of Microbiology & Biotechnology·D Claus
May 28, 2014·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·M Grover, P C Kashyap
Nov 14, 2014·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Yoshinori Umesaki
Dec 9, 2014·Frontiers in Genetics·Nuria SalazarClara G de Los Reyes-Gavilán
Apr 15, 2015·Research in Microbiology·Reet MändarJaak Truu
Jun 3, 2015·Journal of Microbiology and Biotechnology·Maha Al-Asmakh, Fahad Zadjali
Oct 10, 2015·Nutrition in Clinical Practice : Official Publication of the American Society for Parenteral and Enteral Nutrition·Gail A Cresci, Emmy Bawden
Oct 16, 2015·Cellular and Molecular Life Sciences : CMLS·Philippe Gérard
Feb 26, 2016·The International Journal of Neuropsychopharmacology·Pauline LuczynskiJohn F Cryan
Apr 20, 2016·Scientific Reports·Adrien FrançoisNicolas Meunier
May 7, 2016·Methods in Molecular Biology·Yogesh Bhattarai, Purna C Kashyap
Aug 22, 2016·Cellular and Molecular Life Sciences : CMLS·Klara KubelkovaAles Macela
Sep 17, 2016·Endocrinología y nutrición : órgano de la Sociedad Española de Endocrinología y Nutrición·Araceli Muñoz-GarachFrancisco J Tinahones
Dec 10, 2016·Critical Reviews in Microbiology·Lisa F StinsonJeffrey A Keelan
Dec 29, 2016·Allergologia et immunopathologia·Y B KangH Zhang
Apr 1, 2017·Circulation Research·W H Wilson TangStanley L Hazen
Sep 5, 2017·Alimentary Pharmacology & Therapeutics·S R CardingL Hoyles
Dec 30, 2017·Clinical Journal of Gastroenterology·Atsushi NishidaAkira Andoh
Jul 17, 2018·Current Protocols in Immunology·Richard LavinLynn Bry
Nov 16, 2018·Frontiers in Physiology·Elizabeth A KennedyMegan T Baldridge
May 6, 2019·Current Opinion in Pharmacology·Tayfun Uzbay
May 18, 2019·Laboratory Animals·Marijana Basic, André Bleich
Aug 2, 2019·Nature·Marcus C de GoffauGordon C S Smith
Oct 28, 2019·Current Pediatric Reviews·Paola MussoRoberto Bernardini

❮ Previous
Next ❯

Citations

Apr 1, 2021·Microbes and Infection·Meggie KuypersThierry Mallevaey
Oct 30, 2021·Current Opinion in Organ Transplantation·Emmanuel F MongodinJonathan S Bromberg

❮ Previous
Next ❯

Methods Mentioned

BETA
cesarean section
PCR
hysterectomy
light microscopy
electrophoresis

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.