Methods for extracellular vesicles isolation in a hospital setting

Frontiers in Immunology
Matías Sáenz-CuestaDavid Otaegui

Abstract

The research in extracellular vesicles (EVs) has been rising during the last decade. However, there is no clear consensus on the most accurate protocol to isolate and analyze them. Besides, most of the current protocols are difficult to implement in a hospital setting due to being very time-consuming or to requirements of specific infrastructure. Thus, our aim is to compare five different protocols (comprising two different medium-speed differential centrifugation protocols; commercially polymeric precipitation - exoquick - acid precipitation; and ultracentrifugation) for blood and urine samples to determine the most suitable one for the isolation of EVs. Nanoparticle tracking analysis, flow cytometry, western blot (WB), electronic microscopy, and spectrophotometry were used to characterize basic aspects of EVs such as concentration, size distribution, cell-origin and transmembrane markers, and RNA concentration. The highest EV concentrations were obtained using the exoquick protocol, followed by both differential centrifugation protocols, while the ultracentrifugation and acid-precipitation protocols yielded considerably lower EV concentrations. The five protocols isolated EVs of similar characteristics regarding markers and R...Continue Reading

Citations

Jan 3, 2016·Journal of Immunological Methods·Rachel E CrosslandAnne M Dickinson
Apr 23, 2015·International Journal of Molecular Medicine·Rafal SzatanekMonika Baj-Krzyworzeka
Feb 11, 2016·International Journal of Molecular Sciences·Hina KalraSuresh Mathivanan
Feb 3, 2016·Journal of Chromatography. a·Gabriella PocsfalviKároly Vékey
Apr 2, 2016·The Journal of Clinical Investigation·Rong XuRichard J Simpson
Aug 11, 2015·Frontiers in Immunology·Matías Sáenz-CuestaDavid Otaegui
Aug 9, 2016·Biochimica Et Biophysica Acta. General Subjects·Déborah L M RupertMarta Bally
Oct 11, 2016·Methods : a Companion to Methods in Enzymology·Joanne Lannigan, Uta Erdbruegger
Oct 26, 2016·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Roman ŠtukeljRado Janša
Oct 25, 2016·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·M PuhkaT M Af Hällström
Jan 20, 2017·Platelets·Frank A W CoumansRienk Nieuwland
Jul 26, 2016·International Journal of Molecular Sciences·Ainhoa AlberroDavid Otaegui
Dec 16, 2017·Journal of Cellular Biochemistry·Helia NamaziHossein Baharvand
Dec 7, 2017·Frontiers in Chemistry·Livia Rosa-FernandesGiuseppe Palmisano
Jun 12, 2018·PloS One·Tânia Soares MartinsAna Gabriela Henriques
Jul 17, 2019·Current Drug Targets·Abdelrahman Y SherifAbdullah M E Youssof
Jun 8, 2017·Frontiers in Neuroscience·Maja MustapicDimitrios Kapogiannis
Aug 22, 2018·International Journal of Molecular Sciences·Matías Sáenz-CuestaDavid Otaegui
Nov 14, 2019·Metabolites·Charles WilliamsJuan M Falcón-Pérez
Oct 13, 2020·Frontiers in Oncology·Zhe-Ying WangJian-Hua Tong
Jul 2, 2016·Current Opinion in Pharmacology·Veronica TorranoArkaitz Carracedo
Mar 5, 2021·Molecular Psychiatry·Yena LeeRoger S McIntyre
Oct 11, 2019·Trends in Analytical Chemistry : TRAC·Ying ZhangW Andy Tao
May 20, 2021·Neurology. Neuroimmunology and Neuroinflammation·May F MradSamia J Khoury
Aug 8, 2021·International Journal of Molecular Sciences·Ainhoa AlberroDavid Otaegui
Aug 28, 2021·International Journal of Molecular Sciences·Anna TestaMaria Felice Brizzi
Nov 5, 2016·Annual Review of Pharmacology and Toxicology·Milad RiazifarWeian Zhao

❮ Previous
Next ❯

Methods Mentioned

BETA
size-exclusion
chip
blood collection
FACS
flow cytometry
size-exclusion chromatography

Software Mentioned

PASW Statistics
exoquick
NTA
SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.