Methods for histochemical demonstration of vascular structures at the muscle-bone interface from cryostate sections of demineralized tissue

Acta histochemica
S Kirkeby

Abstract

In tissue decalcified with MgNa2EDTA at a neutral pH activity for ATPase can used be for demonstration of the vascular structures at the muscle-bone interface. The GOMORI method for alkaline phosphatase is only of value, when fresh unfixed tissue is to be examined. The azo-dye method for alkaline phosphatase failed to give satisfactory results, and so did the alpha-amylase PAS method. 5'-nucleotidase activity is present in both capillaries and in cells lining the surfaces of bones, while larger blood vessels are poorly stained.

References

Oct 1, 1975·Acta Physiologica Scandinavica·P Andersen
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May 1, 1978·Acta Physiologica Scandinavica·R Myrhage
Feb 1, 1975·Proceedings of the Society for Experimental Biology and Medicine·N Banchero
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May 1, 1965·Archives of Neurology·F C ROMANUL
May 1, 1955·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H A PADYKULA, E HERMAN

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