Methods for in vitro modeling of glioma invasion: Choosing tools to meet the need.

Glia
Bilge DundarDaniel J Brat

Abstract

Widespread tumor cell invasion is a fundamental property of diffuse gliomas and is ultimately responsible for their poor prognosis. A greater understanding of basic mechanisms underlying glioma invasion is needed to provide insights into therapies that could potentially counteract them. While none of the currently available in vitro models can fully recapitulate the complex interactions of glioma cells within the brain tumor microenvironment, if chosen and developed appropriately, these models can provide controlled experimental settings to study molecular and cellular phenomena that are challenging or impossible to model in vivo. Therefore, selecting the most appropriate in vitro model, together with its inherent advantages and limitations, for specific hypotheses and experimental questions achieves primary significance. In this review, we describe and discuss commonly used methods for modeling and studying glioma invasion in vitro, including platforms, matrices, cell culture, and visualization techniques, so that choices for experimental approach are informed and optimal.

References

Apr 24, 2001·Journal of Cancer Research and Clinical Oncology·A Giese, M Westphal
Nov 17, 2001·Biomacromolecules·A I Van Den BulckeH Berghmans
Nov 22, 2001·Journal of Neuro-oncology·Y AkiyamaJ T Rutka
Feb 21, 2003·Glia·Lýdia VargováEva Syková
Apr 3, 2003·International Journal of Cancer. Journal International Du Cancer·Ilunga KalembeyiToshimichi Yoshida
Apr 17, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·A GieseM Westphal
Jan 30, 2004·Proceedings of the National Academy of Sciences of the United States of America·Charles R KeeseIvar Giaever
Apr 7, 2004·Clinical & Experimental Metastasis·Victor OkohRobert I Garver
Feb 3, 2005·Acta Neuropathologica·Hiroko Ohgaki, Paul Kleihues
Nov 18, 2005·Methods : a Companion to Methods in Enzymology·Aline ValsterMarc Symons
Jan 4, 2006·PLoS Computational Biology·Quan Wen, Dmitri B Chklovskii
Aug 9, 2007·Nature Reviews. Molecular Cell Biology·Francesco PampaloniErnst H K Stelzer
Nov 3, 2007·Genes & Development·Frank B FurnariWebster K Cavenee
Nov 7, 2007·Neuropathology and Applied Neurobiology·C MarisI Salmon
Nov 28, 2007·Annual Review of Pathology·David N Louis
Nov 28, 2007·Cancer Letters·Wei WeiJames G McLarnon
Jul 10, 2008·The Journal of Biological Chemistry·Bin HuMariano S Viapiano
Jul 18, 2008·Cancer Research·Mako Narisawa-SaitoTohru Kiyono
Mar 13, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nancy Ann OberheimMaiken Nedergaard
Mar 24, 2009·Neuro-oncology·Adam M BurgoyneSusann M Brady-Kalnay
Oct 20, 2009·Journal of Cell Communication and Signaling·Kim S Midwood, Gertraud Orend
Apr 27, 2010·Biomaterials·Forrest M KievitMiqin Zhang
Nov 6, 2010·Science·R Douglas Fields
Dec 2, 2010·Toxicologic Pathology·Robert H Garman
Jan 12, 2011·Brain Tumor Pathology·Manabu OnishiIsao Date
Mar 23, 2011·Cold Spring Harbor Perspectives in Biology·Peter D Yurchenco
Jul 13, 2011·Methods in Molecular Biology·Giles Cory
Jul 13, 2011·Methods in Molecular Biology·John Marshall
Oct 5, 2011·Neoplasia : an International Journal for Oncology Research·Paula A Agudelo-GarciaMariano S Viapiano
Nov 3, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Michael D ZordanJames F Leary
Jan 3, 2012·Biochimica Et Biophysica Acta·Rolf MentleinJanka Held-Feindt
Sep 4, 2012·Mutation Research·Nina KramerHelmut Dolznig

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