Methods for Investigating the Larval Period and Metamorphosis in Xenopus

Cold Spring Harbor Protocols
Daniel R Buchholz, Yun-Bo Shi

Abstract

Anuran metamorphosis resembles postembryonic development in mammals, a period around birth when many organs/tissues mature into their adult form as circulating thyroid and stress hormone levels are high. Unlike uterus-enclosed mammalian embryos, tadpoles develop externally and undergo the dramatic changes of hormone-dependent development totally independent of maternal influence, making them a valuable model in which to study vertebrate postembryonic organ development and maturation. Various protocols have been developed and/or adapted for studying metamorphosis in Xenopus laevis and X. tropicalis, two highly related and well-studied frog species. Here, we introduce some of the methods for contemporary cell and molecular studies of gene function and regulation during metamorphosis.

References

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Nov 3, 2017·Cold Spring Harbor Protocols·Matthew D PatmannDaniel R Buchholz
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May 18, 2018·Cold Spring Harbor Protocols·Cristina Sáenz de MieraRobert J Denver
Jun 14, 2018·Cold Spring Harbor Protocols·Nicolas BuisineLaurent M Sachs

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