PMID: 7010675Jan 1, 1980Paper

Methods for microcarrier culture of bovine pulmonary artery endothelial cells avoiding the use of enzymes

Tissue & Cell
U S RyanC Whitaker

Abstract

Enzymes situated along the luminal surface of pulmonary endothelial cells interact with circulating solutes, notably with vasoactive substances, to regulate the hormonal composition of systemic arterial blood. However, it is becoming clear that the range and complexity of reactions occurring at or near the surface of endothelial cells are greater than previously recognized. In addition, evidence indicates that the quality of cell cultures used to define specific endothelial functions must be carefully controlled, together with development of improved understanding of the effects of long-term culture on pulmonary endothelial cells. We have developed new techniques for the culture of pulmonary endothelial cells which avoid exposure to proteolytic enzymes at both the isolation step and during subculture. A combination of mechanical harvest and culture on microcarrier beads has provided a system for the long-term, large-scale culture of pulmonary endothelial cells, features which to a large extent determine the scope of biochemical studies which can be undertaken.

References

Jan 1, 1978·Tissue & Cell·U S RyanJ W Ryan
Jun 20, 1978·Annals of the New York Academy of Sciences·E A Jaffe, D F Mosher
Jan 1, 1976·Advances in Experimental Medicine and Biology·U S RyanA Chiu
Mar 28, 1977·Biochimica Et Biophysica Acta·J J Lanzillo, B L Fanburg
Nov 1, 1978·The Journal of Experimental Medicine·H E IvesJ D Jamieson
Jun 1, 1978·The Journal of Experimental Medicine·E A Jaffe, D F Mosher
Dec 1, 1976·Experimental Cell Research·P M Absher, R G Absher
Jul 1, 1975·The Journal of Cell Biology·A G Yee, J P Revel
Dec 1, 1975·The Journal of Cell Biology·M SimionescuG E Palade
Nov 1, 1970·The Biochemical Journal·J W RyanJ G Ledingham
Oct 12, 1971·Biochimica Et Biophysica Acta·J W Ryan, U Smith
Mar 1, 1974·The Journal of Cell Biology·M A GimbroneJ Folkman
Nov 1, 1973·The Journal of Clinical Investigation·E A JaffeR L Nachman
Dec 1, 1968·The Biochemical Journal·J W RyanJ M Stewart

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Citations

Jan 1, 1987·Acta Neuropathologica·O KempskiJ Bembry
Jan 1, 1985·The Journal of Membrane Biology·D M Larson, J D Sheridan
May 1, 1983·In Vitro·C M Gajdusek, S M Schwartz
Mar 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·C M BowmanJ E Repine
Jul 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·K W Wissemann, B S Jacobson
Jan 1, 1985·Tissue & Cell·U S Ryan, L A White
Jan 1, 1986·Tissue & Cell·S C ZhangU S Ryan
Jan 1, 1989·Pharmacology & Therapeutics·U S Ryan, C J Grantham
Aug 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·O ChappeyJ L Wautier
Apr 1, 1997·Cardiovascular Surgery : Official Journal of the International Society for Cardiovascular Surgery·S ItoS E Wilson
Feb 1, 1992·Acta Physiologica Scandinavica·S P Olesen, M Bundgaard
Jul 1, 1989·American Journal of Respiratory Cell and Molecular Biology·H W FarberF L Glauser
Dec 1, 1989·American Journal of Respiratory Cell and Molecular Biology·D B BadeschK R Stenmark
Jul 1, 1992·American Journal of Respiratory Cell and Molecular Biology·G H DownieM Friedman
Jun 1, 1984·Environmental Health Perspectives·U S Ryan
Jan 1, 1992·Journal of Neurochemistry·F Joó
Feb 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D B Stolz, B S Jacobson
Jun 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·R L Vender
Jan 1, 1992·Journal of Physiology, Paris·P AndréJ C Stoclet
Sep 1, 1987·Journal of Vascular Surgery·B E SumpioG Johnson
Jul 28, 2012·Thrombosis and Haemostasis·Jorge CerveróJosé Hermida
Nov 13, 1987·Biochemical and Biophysical Research Communications·D J CrutchleyU S Ryan

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