PMID: 9524450Apr 1, 1997Paper

Methods for microscopic characterization of oral biofilms: analysis of colonization, microstructure, and molecular transport phenomena

Advances in Dental Research
S SingletonC Allison

Abstract

Assessment of the role of biofilm microstructure in biofilm-specific activities requires non-destructive measurement techniques for parameterization of structural characteristics in parallel with relevant biochemical and physiological data. This paper briefly reviews some current methods for biofilm structural analysis, with emphasis on new developments in optical imaging and mathematical modeling methods. Fluorescence imaging studies of bacterial colonization events occurring on exposed model tooth surfaces indicated that bacterial adhesion to sessile organisms was of central importance to the early colonization process and that this occurred in a non-random manner. Structural studies of mature biofilms by confocal microscopy demonstrated the spatial distribution of individual species using fluorescent antibodies. Biofilms grown under different physiological conditions exhibited differences in structure, and methods were developed for parameterizing the spatial orientations of the bacteria. Diffusive processes within biofilm microstructures were studied using a random walk model in both 2-D and 3-D. Modeling of convective flow within biofilm microstructures was achieved by application of lattice Boltzmann methodology.

References

Mar 1, 1977·Journal of Periodontal Research·S S SocranskyJ van Houte
Sep 1, 1978·Journal of Periodontal Research·T Lie
Feb 1, 1992·Applied and Environmental Microbiology·R I AmannD A Stahl
Jul 1, 1992·Antimicrobial Agents and Chemotherapy·H AnwarJ W Costerton
Jan 1, 1991·Biosensors & Bioelectronics·C C Cronenberg, J C van den Heuvel
Mar 1, 1990·Microbiological Reviews·M C van LoosdrechtA J Zehnder
Sep 1, 1987·Reviews of Infectious Diseases·S E MergenhagenJ O Cisar
Oct 1, 1988·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H J GundersenB Pakkenberg
May 1, 1988·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H J GundersenA Vesterby
Jan 1, 1987·Annual Review of Microbiology·J W CostertonT J Marrie
Apr 1, 1994·Journal of Bacteriology·J W CostertonG James
Jun 1, 1993·Journal of Bacteriology·P E Kolenbrander, J London
Dec 29, 1995·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J J BirminghamR Treloar
Dec 1, 1994·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·O BehrendP B Warren
Apr 7, 1986·Physical Review Letters·U FrischY Pomeau
Feb 1, 1994·Applied and Environmental Microbiology·G M WolfaardtD E Caldwell
Apr 1, 1994·Applied and Environmental Microbiology·J R LawrenceD R Korber
Oct 20, 1991·Biotechnology and Bioengineering·Z LewandowskiW G Characklis
Jan 1, 1989·Microbial Ecology·M C van LoosdrechtA J Zehnder

❮ Previous
Next ❯

Citations

Apr 5, 2001·Archives of Oral Biology·T M AuschillN B Artweiler
Jun 11, 1999·Current Opinion in Biotechnology·R J Palmer, C Sternberg
May 5, 2001·Trends in Microbiology·I W Sutherland
Apr 12, 2001·Journal of Applied Microbiology·R J HodgsonC Allison
Mar 7, 2001·Applied and Environmental Microbiology·M GuggenheimB Guggenheim
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·M E Davey, G A O'toole
Sep 21, 2005·Journal of Periodontology·M Robert WirthlinCharles I Hoover
Dec 8, 2010·Periodontology 2000·Philip D MarshDeirdre A Devine
Mar 1, 2002·FEMS Microbiology Ecology·S R WoodC Robinson
Jan 15, 2002·Biotechnology and Bioengineering·Brian D WoodStephen Whitaker
Dec 23, 2011·Brazilian Dental Journal·Fernanda G BassoCarlos A de Souza Costa
Feb 15, 2002·Advances in Dental Research·J D Rudney
May 23, 2007·BioTechniques·Carla C C R de Carvalho, M Manuela R da Fonseca
Aug 26, 2006·Periodontology 2000·Mary E Davey, John W Costerton
Mar 14, 2019·Brazilian Dental Journal·Gabriela RezendeLina Naomi Hashizume
Sep 17, 2019·Journal of Medical Microbiology·Jason L BrownGordon Ramage
Mar 7, 2002·European Journal of Oral Sciences·Thorsten Mathias AuschillLutz Netuschil
Jul 31, 1999·Applied and Environmental Microbiology·J M VroomC Allison

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.