Methods for observing microbial biofilms directly on leaf surfaces and recovering them for isolation of culturable microorganisms.

Applied and Environmental Microbiology
C E MorrisM A Jacques

Abstract

Epifluorescence microscopy, scanning electron microscopy, and confocal laser scanning microscopy were used to observe microbial biofilms directly on leaf surfaces. Biofilms were observed on leaves of all species sampled (spinach, lettuce, Chinese cabbage, celery, leeks, basil, parsley, and broad-leaved endive), although the epifluorescent images were clearest when pale green tissue or cuticle pieces were used. With these techniques, biofilms were observed that were about 20 (mu)m in depth and up to 1 mm in length and that contained copious exopolymeric matrices, diverse morphotypes of microorganisms, and debris. The epifluorescence techniques described here can be used to rapidly determine the abundance and localization of biofilms on leaves. An additional technique was developed to recover individual biofilms or portions of single biofilms from leaves and to disintegrate them for isolation of the culturable microorganisms they contained. Nineteen biofilms from broad-leaved endive, spinach, parsley, and olive leaves were thus isolated and characterized to illustrate the applications of this technique.

References

Jun 1, 1992·Applied and Environmental Microbiology·G G RodriguezH F Ridgway
Jan 1, 1987·Annual Review of Microbiology·J W CostertonT J Marrie
Jun 1, 1995·Applied and Environmental Microbiology·C T HuangP S Stewart
Oct 1, 1994·International Journal of Food Microbiology·E A Zottola, K C Sasahara
Jul 1, 1994·Applied and Environmental Microbiology·F P Yu, G A McFeters
Jan 1, 1995·Annual Review of Microbiology·J W CostertonH M Lappin-Scott
Jan 1, 1994·Journal of Microbiological Methods·F P Yu, G A McFeters
Jul 1, 1984·Applied and Environmental Microbiology·R W RobinsonH C Aldrich
Sep 1, 1994·Applied and Environmental Microbiology·D A BrownT J Beveridge
Dec 1, 1994·Applied and Environmental Microbiology·M Wilson, S E Lindow
Jan 1, 1995·Annual Review of Phytopathology·G A Beattie, S E Lindow

❮ Previous
Next ❯

Citations

Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·M E Davey, G A O'toole
Jan 10, 2001·Current Opinion in Urology·M Sofer, J D Denstedt
Nov 10, 2001·Annual Review of Phytopathology·John H Andrews, Robin F Harris
Jan 5, 2002·Journal of Food Protection·K Takeuchi, J F Frank
Apr 25, 2003·Journal of Biosciences·Gamini Seneviratne, H S Jayasinghearachchi
May 6, 2003·Annual Review of Phytopathology·Cindy E Morris, Jean-Michel Monier
Jan 9, 2004·Applied and Environmental Microbiology·J-M Monier, S E Lindow
Sep 10, 2005·Applied and Environmental Microbiology·J-M Monier, S E Lindow
May 5, 2005·Applied and Environmental Microbiology·Brendan A Niemira, Ethan B Solomon
May 18, 2006·Annual Review of Phytopathology·Maria T Brandl
Oct 24, 2008·Phytopathology·G A Beattie, S E Lindow
Jan 1, 1997·Annual Review of Phytopathology·L L Kinkel
Feb 3, 2007·Journal of Food Protection·Montserrat H IturriagaEduardo F Escartín
Dec 1, 1998·Journal of Applied Microbiology·I CarmichaelM W Hickey
Mar 29, 2011·Annual Review of Phytopathology·Gwyn A Beattie
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·J H Leveau, S E Lindow
Jun 24, 2008·Critical Reviews in Food Science and Nutrition·Vicente M Gómez-LópezFrank Devlieghere
Oct 6, 2000·Journal of Applied Microbiology·K BjörklöfM Romantschuk
May 15, 2004·Molecular Plant-microbe Interactions : MPMI·Beatriz QuiñonesSteven E Lindow
Mar 1, 2012·Environmental Microbiology·Robin Tecon, Johan H J Leveau
Sep 8, 2006·Applied and Environmental Microbiology·Molly J McGrath, John H Andrews
Aug 28, 2020·Frontiers in Cellular and Infection Microbiology·Lasse KvichMads Lichtenberg
Apr 2, 1999·Journal of Endourology·G ReidJ Denstedt
Apr 5, 2003·Applied and Environmental Microbiology·Steven E Lindow, Maria T Brandl
Apr 4, 2003·Canadian Journal of Microbiology·William F Fett, Peter H Cooke
Feb 10, 2009·Journal of Food Science·Bassam A AnnousJames L Smith
Jun 10, 2015·Virulence·Janaina de Cássia Orlandi SardiMaria Jose Soares Mendes Giannini

❮ Previous
Next ❯

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.