Methods for production of monoclonal antibodies with specificity for human lung cancer cells

In Vitro
J D MinnaS Krasnow

Abstract

We have developed a screening strategy and technology to produce monoclonal antibodies with specificity for human lung cancer cells. Mice and rats were immunized with well-characterized tissue culture lines of human small cell lung cancer (SCLC), mouse myeloma x spleen hybrids formed by the technique of Kohler and Milstein, and the resulting culture fluids were screened for antibody binding phenotype using a radioimmunoassay. To facilitate testing large numbers of culture fluids, a 96-well, microtiter based, reusable, replicating device was designed. Using this, many hybridoma culture fluids were replica plated for antibody binding tests on a series of human target cell plates. Hybrids producing antibodies that reacted with the immunizing SCLC line and another independent SCLC line, but not with autologous B-lymphoblastoid cells derived from one of the patients, were identified, selected, and then repeatedly recloned using the same screening strategy. With this technology, hybridomas representing less than 0.5% of all hybrids generated could be isolated and stable antibody producing cultures derived. Such antibodies reacted with a panel of well-characterized SCLC lines and SCLC samples taken directly from patients but not with ...Continue Reading

References

Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H KoprowskiM Herlyn
Jan 15, 1976·International Journal of Cancer. Journal International Du Cancer·M LieberG Todaro
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M Y YehK E Hellström
Jan 1, 1979·Journal of Immunological Methods·J P BrownI Hellström
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R LevyR Warnke
Apr 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·M C WeissL J Scaletta
Nov 1, 1973·Journal of the National Cancer Institute·H D SouleM Brennan
Jan 1, 1966·Experimental Cell Research·J W Littlefield
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L Olsson, H S Kaplan
Apr 3, 1981·Science·H KoprowskiH F Sears
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M HsuH Fanger
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D N CarneyJ D Minna
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·F CuttittaJ D Minna
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·W G DippoldL J Old
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J SchlomY A Teramoto
Mar 12, 1981·Nature·S Olsnes
Jan 1, 1981·International Journal of Immunopharmacology·M Belles-Isles, M Pagé

❮ Previous
Next ❯

Citations

Apr 1, 1984·Clinical Biochemistry·J Shuster
Oct 1, 1988·European Journal of Cancer & Clinical Oncology·J BenardG Riou
Jun 15, 1985·International Journal of Cancer. Journal International Du Cancer·J G ReeveN M Bleehen
Mar 1, 1985·Postgraduate Medicine·S T RosenA L Epstein

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.