Listeria monocytogenes is an important food-borne pathogen and is widely tested for in food, environmental and clinical samples. Identification traditionally involved culture methods based on selective enrichment and plating followed by the characterization of Listeria spp. based on colony morphology, sugar fermentation and haemolytic properties. These methods are the gold standard; but they are lengthy and may not be suitable for testing of foods with short shelf lives. As a result more rapid tests were developed based on antibodies (ELISA) or molecular techniques (PCR or DNA hybridization). While these tests possess equal sensitivity, they are rapid and allow testing to be completed within 48 h. More recently, molecular methods were developed that target RNA rather than DNA, such as RT-PCR, real time PCR or nucleic acid based sequence amplification (NASBA). These tests not only provide a measure of cell viability but they can also be used for quantitative analysis. In addition, a variety of tests are available for sub-species characterization, which are particularly useful in epidemiological investigations. Early typing methods differentiated isolates based on phenotypic markers, such as multilocus enzyme electrophoresis, pha...Continue Reading
Detection of genes coding for listeriolysin and Listeria monocytogenes antigen A (ImaA) in Listeria spp. by the polymerase chain reaction
Restriction fragment length polymorphism in four virulence-associated genes of Listeria monocytogenes
Use of specific oligonucleotides for direct enumeration of Listeria monocytogenes in food samples by colony hybridization and rapid detection by PCR
A comparative study of randomly amplified polymorphic DNA analysis and conventional phage typing for epidemiological studies of Listeria monocytogenes isolates
Suitability of the prfA gene, which encodes a regulator of virulence genes in Listeria monocytogenes, in the identification of pathogenic Listeria spp.
Phosphatidylinositol-specific phospholipase C activity as a marker to distinguish between pathogenic and nonpathogenic Listeria species.
A rapid polymerase chain reaction (PCR)-based assay for the identification of Listeria monocytogenes in food samples
Detection and identification of Listeria monocytogenes in cooked sausage products and in milk by in vitro amplification of haemolysin gene fragments
Listeria monocytogenes isolates can be classified into two major types according to the sequence of the listeriolysin gene.
The gene coding for protein p60 of Listeria monocytogenes and its use as a specific probe for Listeria monocytogenes.
Analysis of clinical and food-borne isolates of Listeria monocytogenes in the United States by multilocus enzyme electrophoresis and application of the method to epidemiologic investigations.
Identification of a gene that positively regulates expression of listeriolysin, the major virulence factor of listeria monocytogenes
The evaluation of a phage-typing system for Listeria monocytogenes for use in epidemiological studies
Aspects of the epidemiology of human Listeria monocytogenes infections in Britain 1967-1984; the use of serotyping and phage typing
Listeria monocytogenes exists in at least three evolutionary lines: evidence from flagellin, invasive associated protein and listeriolysin O genes
Study of Listeria monocytogenes contamination in a dairy plant and characterization of the strains isolated
PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid.
Enhanced sensitivity in PCR detection of Listeria monocytogenes in soft cheese through use of an aqueous two-phase system as a sample preparation method.
Enrichment in Fraser broth supplemented with catalase or Oxyrase, combined with the microcolony immunoblot technique, for detecting heat-injured Listeria monocytogenes in foods
Differential activation of virulence gene expression by PrfA, the Listeria monocytogenes virulence regulator
Contamination pattern of Listeria monocytogenes and other Listeria spp. in a salmon slaughterhouse and smoked salmon processing plant
Comparison of multilocus enzyme electrophoresis (MEE), ribotyping, restriction enzyme analysis (REA) and phage typing for typing of Listeria monocytogenes
Detection of Campylobacter jejuni added to foods by using a combined selective enrichment and nucleic acid sequence-based amplification (NASBA).
Typing Listeria monocytogenes: a comparison of random amplification of polymorphic DNA with 5 other methods
Rapid RAPD analysis for distinguishing Listeria species and Listeria monocytogenes serotypes using a capillary air thermal cycler
Characterization of Listeria monocytogenes isolated from poultry products and from the poultry-processing environment by random amplification of polymorphic DNA and multilocus enzyme electrophoresis.
Incidence of Listeria spp. and Listeria monocytogenes in a poultry processing environment and in poultry products and their rapid confirmation by multiplex PCR.
Comparison of ribotyping and multilocus enzyme electrophoresis for subtyping of Listeria monocytogenes isolates.
Proposals for optimization of the international phage typing system for Listeria monocytogenes: combined analysis of phage lytic spectrum and variability of typing results.
Direct detection of Listeria monocytogenes using paramagnetic bead DNA extraction and enzymatic DNA amplification
Identification of five Listeria species based on infrared spectra (FTIR) using macrosamples is superior to a microsample approach
Development and validation of qualitative SYBR®Green real-time PCR for detection and discrimination of Listeria spp. and Listeria monocytogenes.
A quantitative real-time PCR assay for quantification of viable Listeria monocytogenes cells after bacteriocin injury in food-first insights.
Multiplex PCR for the identification and serotyping of L. monocytogenes isolated from sheep cheese-processing plants
Comparison of conventional and molecular methods for the routine confirmation of Listeria monocytogenes in milk products produced domestically in Croatia
A review of the ecology, genomics, and stress response of Listeria innocua and Listeria monocytogenes
Evaluation of effects of primary and secondary enrichment for the detection of Listeria monocytogenes by real-time PCR in retail ground chicken meat
Prevalence of Listeria in Chinese food products from 13 provinces between 2000 and 2007 and virulence characterization of Listeria monocytogenes isolates
Distribution and characteristics of Listeria monocytogenes isolates from surface waters of the South Nation River watershed, Ontario, Canada
Real-time PCR assay to differentiate Listeriolysin S-positive and -negative strains of Listeria monocytogenes
Stress- and growth rate-related differences between plate count and real-time PCR data during growth of Listeria monocytogenes.
Truncated human cytidylate-phosphate-deoxyguanylate-binding protein for improved nucleic acid amplification technique-based detection of bacterial species in human samples.
A comparison of enrichment and direct-plating methods for isolation of Listeria monocytogenes from surface water
Vascular Endograft Infection with Listeria monocytogenes reated with Surgical Debridement but without Graft Removal
Highly specific fiber optic immunosensor coupled with immunomagnetic separation for detection of low levels of Listeria monocytogenes and L. ivanovii
Specific and Rapid Quantification of Viable Listeria monocytogenes Using Fluorescence in situ Hybridization in Combination with Filter Cultivation
An alternative chemical redox method for the production of bispecific antibodies: implication in rapid detection of food borne pathogens
Establishment of a simple and rapid identification method for Listeria spp. by using high-resolution melting analysis, and its application in food industry
Evaluation of immunochromatography for the rapid and specific identification of Listeria monocytogenes from food
Rapid identification and source-tracking of Listeria monocytogenes using MALDI-TOF mass spectrometry
How the interaction of Listeria monocytogenes and Acanthamoeba spp. affects growth and distribution of the food borne pathogen
Estimating the diagnostic accuracy of three culture-dependent methods for the Listeria monocytogenes detection from a Bayesian perspective
Recent advances in molecular technologies and their application in pathogen detection in foods with particular reference to yersinia.
Detection of Listeria monocytogenes from selective enrichment broth using MALDI-TOF Mass Spectrometry
Evaluation of reduction of Fraser incubation by 24h in the EN ISO 11290-1 standard on detection and diversity of Listeria species
Rapid detection of Listeria monocytogenes in milk using confocal micro-Raman spectroscopy and chemometric analysis
Rapid Detection of Listeria by Bacteriophage Amplification and SERS-Lateral Flow Immunochromatography
Molecular methods to assess Listeria monocytogenes route of contamination in a dairy processing plant
Evaluation of immunomagnetic separation in combination with ALOA Listeria chromogenic agar for the isolation and identification of Listeria monocytogenes in ready-to-eat foods
Quantification of Listeria monocytogenes in minimally processed leafy vegetables using a combined method based on enrichment and 16S rRNA real-time PCR
Listeria monocytogenes in RTE foods marketed in Italy: prevalence and automated EcoRI ribotyping of the isolates
Detection, quantification and vitality of Listeria monocytogenes in food as determined by quantitative PCR
An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food
Rapid real-time PCR detection of Listeria monocytogenes in enriched food samples based on the ssrA gene, a novel diagnostic target
Construction and evaluation of a microbiological positive process internal control for PCR-based examination of food samples for Listeria monocytogenes and Salmonella enterica
Prevalence of Listeria monocytogenes in fresh and fermented Italian sausages and ribotyping of contaminating strains
Occurrence and persistence of Listeria spp. in the environment of ewe and cow's milk cheese dairies in Portugal unveiled by an integrated analysis of identification, typing and spatial-temporal mapping along production cycle
Evaluation of indirect and avidin-biotin enzyme linked immunosorbent assays for detection of anti-listeriolysin O antibodies in bovine milk samples
Bacteriophage: Phage Therapy
Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.