PMID: 2093626Jan 1, 1990Paper

Methods of drug protein binding determinations

Fundamental & Clinical Pharmacology
B Sebille

Abstract

The determination of drug binding to proteins is carried out by several different approaches based mainly on separation methods. The most popular of these is equilibrium dialysis, which requires membranes devoid of non-specific adsorption to drugs. Ultrafiltration techniques have some advantage over dialysis due to shorter measurement times and the possibility of evaluation of the free drug under circumstances corresponding to physiological conditions. Now there is an increasing use of chromatographic techniques for fundamental studies on determination of binding parameters (number of sites, affinity constant). Different procedures can be chosen depending upon the biological materials available. They are based on the presence of the interacting species in the eluent, avoiding any chemical grafting.

References

Apr 15, 1978·Archives of Biochemistry and Biophysics·J A SophianopoulosW P Cropper
Dec 1, 1989·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·L SoltésD Berek
Oct 24, 1986·Journal of Chromatography·S E Cook, T C Pinkerton
Dec 16, 1988·Journal of Chromatography·I MarleT Arvidsson
Jan 1, 1984·Clinical Pharmacokinetics·R H Levy, T A Moreland
Jan 1, 1984·Clinical Pharmacokinetics·W F BowersJ Thompson
Feb 1, 1981·Journal of Pharmaceutical Sciences·J B Whitlam, K F Brown
Oct 8, 1962·Biochimica Et Biophysica Acta·J P HUMMEL, W J DREYER

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Citations

Apr 10, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ladislav Soltés, Mojmír Mach
May 4, 2011·Antimicrobial Agents and Chemotherapy·Markus A ZeitlingerUrsula Theuretzbacher
Apr 25, 2003·Journal of Pharmaceutical Sciences·Michael J BankerJohn A Williams
Jun 26, 2014·Journal of Pharmaceutical Sciences·Kim VanstraelenIsabel Spriet
Jun 17, 2010·Journal of Neural Engineering·Saso JezernikManfred Morari
Apr 23, 2008·Current Protocols in Protein Science·J S Philo
Oct 12, 1999·The American Journal of Physiology·J DupuisC A Goresky
Nov 21, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·J Gailer, W Lindner
Feb 23, 1996·Journal of Chromatography. B, Biomedical Applications·J OravcováW Lindner
May 11, 2021·The Journal of Antimicrobial Chemotherapy·Beatrix WulkersdorferMarkus Zeitlinger
Jan 30, 2020·Journal of Medicinal Chemistry·Gergő DargóGyörgy T Balogh

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