Methods of isolating extracellular vesicles impact down-stream analyses of their cargoes

Methods : a Companion to Methods in Enzymology
Douglas D Taylor, Sahil Shah

Abstract

Viable tumor cells actively release vesicles into the peripheral circulation and other biologic fluids, which exhibit proteins and RNAs characteristic of that cell. Our group demonstrated the presence of these extracellular vesicles of tumor origin within the peripheral circulation of cancer patients and proposed their utility for diagnosing the presence of tumors and monitoring their response to therapy in the 1970s. However, it has only been in the past 10 years that these vesicles have garnered interest based on the recognition that they serve as essential vehicles for intercellular communication, are key determinants of the immunosuppressive microenvironment observed in cancer and provide stability to tumor-derived components that can serve as diagnostic biomarkers. To date, the clinical utility of extracellular vesicles has been hampered by issues with nomenclature and methods of isolation. The term "exosomes" was introduced in 1981 to denote any nanometer-sized vesicles released outside the cell and to differentiate them from intracellular vesicles. Based on this original definition, we use "exosomes" as synonymous with "extracellular vesicles." While our original studies used ultracentrifugation to isolate these vesicles...Continue Reading

References

Jan 15, 1979·International Journal of Cancer. Journal International Du Cancer·W J van BlitterswijkC A Feltkamp
Jul 6, 1981·Biochimica Et Biophysica Acta·E G TramsU Heine
Oct 16, 2002·Journal of Immunological Methods·Henry G LamparskiJean-Bernard Le Pecq
May 24, 2005·International Immunology·Marie-Pierre CabyChristian Bonnerot
Feb 17, 2009·Analytica Chimica Acta·Barbara RodaAldo Roda
Oct 8, 2009·Proteomics·Suresh Mathivanan, Richard J Simpson
Jun 21, 2011·Seminars in Immunopathology·Douglas D Taylor, Cicek Gercel-Taylor
Jun 14, 2012·Analytical Biochemistry·Cicek Gercel-TaylorDouglas D Taylor
Jul 10, 2012·Nucleic Acids Research·Jaime PalmaDominik M Duelli
Oct 12, 2012·PloS One·Deyi XiaoKelly M McMasters
Sep 7, 2013·Journal of Extracellular Vesicles·Kenneth W WitwerFred Hochberg
Mar 29, 2014·Journal of Extracellular Vesicles·Aleksander CvjetkovicCecilia Lässer

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Citations

Feb 7, 2016·Journal of Proteomics·Maurizio BruschiIsabella Panfoli
Dec 30, 2015·International Journal of Molecular Sciences·Edna M MoraElisa Oltra
Feb 19, 2016·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Elena V Batrakova, Myung Soo Kim
Feb 16, 2016·The Journal of Steroid Biochemistry and Molecular Biology·Hanne Roberg-LarsenSteven Ray Wilson
Jan 3, 2016·Journal of Immunological Methods·Rachel E CrosslandAnne M Dickinson
Feb 11, 2016·International Journal of Molecular Sciences·Hina KalraSuresh Mathivanan
Feb 3, 2016·Journal of Chromatography. a·Gabriella PocsfalviKároly Vékey
Jul 19, 2016·Current Molecular Biology Reports·Jess MorhayimMarjolein van Driel
Oct 31, 2016·Advanced Drug Delivery Reviews·Pieter VaderRaymond M Schiffelers
Aug 5, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Elena V Batrakova, Myung Soo Kim
Dec 15, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Norberto A Guzman, Daniel E Guzman
Mar 29, 2016·Journal of Extracellular Vesicles·Chang-Sook HongTheresa L Whiteside
Oct 5, 2015·Cytotechnology·Johann Mar GudbergssonMeg Duroux
Feb 19, 2016·Journal of Alzheimer's Disease : JAD·Robert A SternDouglas D Taylor
Apr 5, 2016·Expert Review of Molecular Diagnostics·Michael Boyiadzis, Theresa L Whiteside
Oct 30, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jennifer F BargerS Patrick Nana-Sinkam
Jun 25, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Lufen HuangLiang Liu
Jul 5, 2016·European Journal of Pharmacology·Stefania BrunoBenedetta Bussolati
Jul 12, 2016·International Journal of Urology : Official Journal of the Japanese Urological Association·Che-Ming LiuShian-Ying Sung
Jul 31, 2016·Journal of Cell Communication and Signaling·Arvind SubramanianSushanta K Banerjee
Aug 16, 2016·Clinics in Laboratory Medicine·Lindsay N Strotman, Mark W Linder
Jul 28, 2016·Radiation Research·Stéphane Flamant, Radia Tamarat
Sep 2, 2016·Stem Cell Research & Therapy·Jeffrey L SpeesCarl A Gregory
Aug 30, 2016·International Journal of Molecular Sciences·Lorena UrbanelliCarla Emiliani
Aug 12, 2015·Methods : a Companion to Methods in Enzymology·Douglas D Taylor
Aug 16, 2016·Biosensors & Bioelectronics·Myriam Oliveira-RodríguezM C Blanco-López
Sep 30, 2016·Cell Communication and Signaling : CCS·Stephan M Feller, Marc Lewitzky
Aug 20, 2016·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Shuangpeng CaiJun Li
Aug 10, 2016·Scientific Reports·Yong Hyun ParkJi Youl Lee
Apr 14, 2016·Scientific Reports·Mark A RiderDavid G Meckes
Jun 27, 2017·The Journal of Investigative Dermatology·Jeffrey D McBrideEvangelos V Badiavas
Apr 8, 2017·Journal of Extracellular Vesicles·Kristine BlansJan T Rasmussen
Jun 27, 2017·Nature Reviews. Microbiology·Nancy Raab-Traub, Dirk P Dittmer
Jul 4, 2017·ACS Nano·Stuart D IbsenMichael J Heller
Dec 14, 2017·Chembiochem : a European Journal of Chemical Biology·Imola Cs SzigyártóTamás Beke-Somfai
Feb 28, 2018·The AAPS Journal·Sihan WangZhenjia Wang
May 2, 2018·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Birke J BenedikterFrank R M Stassen
Jan 27, 2018·Journal of Extracellular Vesicles·Pablo García-ManriqueMaría Carmen Blanco-López
Feb 8, 2018·Journal of Extracellular Vesicles·Roman KornilovSippy Kaur

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