Methods to crystallize RNA

Current Protocols in Nucleic Acid Chemistry
Adrian R Ferré-D'Amaré, Jennifer A Doudna

Abstract

Preparation of suitably large and well-ordered single crystals is usually the rate-limiting step in the determination of the three-dimensional structure of RNAs and their complexes with proteins by X-ray crystallography. This unit discusses a variety of experimental considerations for obtaining crystals of RNAs and RNA-protein complexes. Topics include design of crystallizable constructs, screening, and optimization of crystallization conditions.

References

Mar 8, 1979·Nature·R W SchevitzJ L Sussman
Oct 25, 1992·Nucleic Acids Research·M Puttaraju, M D Been
Apr 20, 1990·European Journal of Biochemistry·A McPherson
May 1, 1988·Quarterly Reviews of Biophysics·J DubochetP Schultz
Jan 1, 1985·Methods in Enzymology·J S Richardson, D C Richardson
Jan 1, 1985·Methods in Enzymology·S R Holbrook, S H Kim
Jan 1, 1985·Methods in Enzymology·G A Petsko
Dec 14, 1972·Journal of Molecular Biology·J E LadnerB F Clark
Aug 16, 1974·Nature·J D RobertusA Klug
Apr 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·S H KimA Rich
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Dec 14, 1993·Biochimica Et Biophysica Acta·J BrattyR Cedergren
Jun 9, 1995·Journal of Molecular Biology·H SchindelinU Heinemann
Jan 1, 1995·Nature Structural Biology·K J BaeyensS R Holbrook
Nov 3, 1994·Nature·H W PleyD B McKay
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·R E DickersonS Neidle
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J A DoudnaC E Kundrot
Jun 21, 1996·Journal of Molecular Biology·A C AndersonC A Frederick
Sep 15, 1996·Structure·S L Cohen
Dec 1, 1995·Nature Structural Biology·S W Wukovitz, T O Yeates
Jan 1, 1997·Biopolymers·S R Holbrook, S H Kim
Jan 1, 1997·Methods in Molecular Biology·A R Ferré-d'Amaré, J A Doudna
Jan 1, 1996·Progress in Biophysics and Molecular Biology·H M BermanJ Westbrook
Nov 14, 1997·Structure·N E Chayen
Jun 26, 1998·Journal of Molecular Biology·A R Ferré-D'AmaréJ A Doudna
Aug 4, 1998·Structure·J L Smith, A Thompson
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·J A Ippolito, T A Steitz
Oct 8, 1998·Acta Crystallographica. Section D, Biological Crystallography·J M HarpG J Bunick
Oct 23, 1998·Nature·A R Ferré-D'AmaréJ A Doudna
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·C C CorrellT A Steitz

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Citations

Jun 2, 2016·Current Protocols in Nucleic Acid Chemistry·Martin Egli
Oct 24, 2019·BMC Bioinformatics·Marcin MagnusJanusz M Bujnicki

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