Methods to study differences in cell mobility during skin wound healing in vitro

Journal of Biomechanics
Hanneke N MonsuurSusan Gibbs

Abstract

Wound healing events which occur in humans are difficult to study in animals due to differences in skin physiology. Furthermore there are increasing restrictions in Europe for using animals for testing the therapeutic properties of new compounds. Therefore, in line with the 3Rs (reduction, refinement and replacement of test animals), a number of human in vitro models of different levels of complexity have been developed to investigate cell mobility during wound healing. Keratinocyte, melanocyte, fibroblast and endothelial cell mobility are described, since these are the residential cells which are responsible for restoring the main structural features of the skin. A monolayer scratch assay is used to study random fibroblast and endothelial cell migration in response to EGF and bFGF respectively and a chemotactic assay is used to study directional fibroblast migration towards CCL5. In order to study endothelial sprouting in response to bFGF or VEGF, which involves continuous degradation and resynthesis of a 3D matrix, a fibrin gel is used. Human physiologically relevant tissue-engineered skin models are used to investigate expansion of the stratified, differentiated epidermis (keratinocytes and melanocytes) over a fibroblast pop...Continue Reading

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Citations

Jan 26, 2017·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Sara Ud-Din, Ardeshir Bayat
Apr 8, 2017·Journal of Cellular Physiology·Jeroen K BuskermolenSusan Gibbs
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Jun 26, 2021·Journal of Biomedical Nanotechnology·Wei JiangShuyan Zhao
Sep 23, 2021·Journal of Tissue Engineering and Regenerative Medicine·Shou Jin PhangMee Lee Looi

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