Methods to study pulmonary dendritic cell migration

Methods in Molecular Biology
Claudia Jakubzick, Gwendalyn J Randolph

Abstract

Dendritic cell migration from the airway to the lymph nodes is a key event in the development of airway immunity during infection, allergy, and vaccination. With judicial selection of materials, there are two approaches to study dendritic cell migration to the mediastinal lymph nodes without the induction of inflammation: airway administration of fluorescent OVA and latex beads. Our protocol describes how to label and track pulmonary dendritic cells from the airway and lung to the mediastinal lymph nodes and reveals how to avoid pitfalls and suboptimal assays.

Citations

Aug 24, 2011·The Journal of Experimental Medicine·A Nicole DeschClaudia V Jakubzick
Aug 4, 2012·Journal of Aerosol Medicine and Pulmonary Drug Delivery·Elena ProiettiPhilipp Latzin
Jul 8, 2010·Blood·Karel OteroSilvano Sozzani
Aug 7, 2013·Proceedings of the National Academy of Sciences of the United States of America·Iris K PangAkiko Iwasaki
Apr 25, 2017·Nature Reviews. Immunology·Claudia V JakubzickPeter M Henson
Aug 20, 2014·Nature Communications·A Nicole DeschClaudia Jakubzick
Sep 13, 2012·Immunologic Research·A Nicole DeschClaudia V Jakubzick
Nov 22, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maria IolyevaCornelia Halin
Nov 18, 2018·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·Steve GeorasJon Meddings
Nov 23, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·César Muñoz-FontelaStuart A Aaronson
Jan 24, 2015·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J L AtherM E Poynter
Oct 6, 2012·The Journal of Allergy and Clinical Immunology·Junyan HanErwin W Gelfand

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.