Dec 5, 2019

Methods to Visualize Auxin and Cytokinin Signaling Activity in the Shoot Apical Meristem

Methods in Molecular Biology
Géraldine BrunoudTeva Vernoux

Abstract

Visualizing the distribution of hormone signaling activity such as auxin and cytokinins is of key importance for understanding regulation of plant development and physiology. Live imaging and genetically encoded hormone biosensors and reporters allow monitoring the spatial and temporal distribution of these phytohormones. Here, we describe how to cultivate live shoot apical meristems after dissection for observation under the confocal microscope for up to 4 days. The shoot apical meristems are maintained on an appropriate medium allowing them to grow and initiate new organs at a frequency similar to plants grown on soil. Meristems expressing hormone biosensors and reporters allows following hormone signaling activity distribution at high spatiotemporal resolution without chemical fixation, an approach that that can also be applied to follow the dynamics of expression in vivo of any fluorescent marker.

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Mentioned in this Paper

Biological Markers
In Vivo
Spatial Distribution
Auxins
Organ
Apical
Cytokinins
Monitoring - Action
Tissue Dissection
Physiological Aspects

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