Methothrexate attenuates early neutrophil infiltration and the associated lipid peroxidation in the injured spinal cord but does not induce neurotoxicity in the uninjured spinal cord in rats

Acta neurochirurgica
Ahmet Metin SanliZeki Sekerci

Abstract

The goal of most acute therapies for spinal cord injury (SCI) in humans include attenuation of the early inflammatory response and may limit the extent of tissue injury and the consequent disability. The purpose of this study was to investigate the early effects of methothrexate (MTX) treatment on myeloperoxidase (MPO) activity, malondialdehyde (MDA) level, and ultrastructural findings in the injured and uninjured spinal cords of rats. The effects of MTX treatment were also compared with methylprednisolone sodium succinate (MPSS) treatment. Wistar rats were divided into seven groups: control; trauma alone (50 g/cm weight drop trauma); SCI + MPSS (30 mg/kg); SCI + low-dose (0.5 mg/kg) MTX (LDMTX); SCI + higher-dose (1 mg/kg) MTX (HDMTX); non-trauma + LDMTX; non-trauma + HDMTX. Administration of MTX and MPSS treatments significantly decreased MPO activity (p < 0.05) and MDA level (p < 0.05) in the first 24 h. The MTX treatments, particularly HDMTX, were more effective than MPSS in reducing MPO activity, and MTX treatments were also more effective than MPSS in reducing MDA level (p < 0.05). The MTX treatment was more protective on large- and medium-diameter myelinated axons in minimizing ultrastructural changes in the spinal-cord-...Continue Reading

References

May 1, 1978·Analytical Biochemistry·M Mihara, M Uchiyama
Nov 3, 1983·The New England Journal of Medicine·J JolivetB A Chabner
Aug 1, 1997·Neuroscience·Y TaokaK Takatsuki
Aug 11, 1998·Journal of Neuroscience Research·D M McTigueB T Stokes
Jul 6, 2000·Immunopharmacology·L GenestierJ P Revillard
Oct 18, 2001·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·A LugaresiD Gambi
Jun 5, 2002·Current Opinion in Neurology·John R Bethea, W Dalton Dietrich
Jul 18, 2002·Journal of Neurosurgery·Erkan KaptanogluEtem Beskonakli
Jun 20, 2003·Spinal Cord·O N Hausmann
Jan 24, 2004·The Journal of Pharmacy and Pharmacology·Kouya YamakiShin Yoshino
May 28, 2004·Pharmacotherapy·Stacia Brock, Heath R Jennings
Feb 18, 2005·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·Edward D Hall, Joe E Springer
Oct 31, 2006·Brain : a Journal of Neurology·Jennifer C FlemingLynne C Weaver
Mar 16, 2007·Journal of Neuropathology and Experimental Neurology·Tiffany RiceV Wee Yong
Dec 7, 2007·Clinical Neuroscience Research·Alpa TrivediLinda J Noble-Haeusslein
Mar 21, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jin-Moo LeeJan Xu
Jun 8, 2010·Journal of Neurochemistry·Gabrièle PiatonCatherine Lubetzki
Jun 10, 2010·Journal of Neurology·Saud A SadiqLauren M Puccio
Aug 31, 2010·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·A Metin SanliZeki Sekerci
Dec 24, 2010·Therapeutic Advances in Neurological Disorders·Bernd C Kieseier, Douglas R Jeffery

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