Methotrexate-Loaded Four-Arm Star Amphiphilic Block Copolymer Elicits CD8+ T Cell Response against a Highly Aggressive and Metastatic Experimental Lymphoma

ACS Applied Materials & Interfaces
Sumit Kumar HiraPartha Pratim Manna

Abstract

We have synthesized a well-defined four-arm star amphiphilic block copolymer [poly(DLLA)-b-poly(NVP)]4 [star-(PDLLA-b-PNVP)4] that consists of D,L-lactide (DLLA) and N-vinylpyrrolidone (NVP) via the combination of ring-opening polymerization (ROP) and xanthate-mediated reversible addition-fragmentation chain transfer (RAFT) polymerization. Synthesis of the polymer was verified by 1H NMR spectroscopy and gel permeation chromatography (GPC). The amphiphilic four-arm star block copolymer forms spherical micelles in water as demonstrated by transmission electron microscopy (TEM) and 1H NMR spectroscopy. Pyrene acts as a probe to ascertain the critical micellar concentration (cmc) by using fluorescence spectroscopy. Methotrexate (MTX)-loaded polymeric micelles of star-(PDLLA15-b-PNVP10)4 amphiphilic block copolymer were prepared and characterized by fluorescence and TEM studies. Star-(PDLLA15-b-PNVP10)4 copolymer was found to be significantly effective with respect to inhibition of proliferation and lysis of human and murine lymphoma cells. The amphiphilic block copolymer causes cell death in parental and MTX-resistant Dalton lymphoma (DL) and Raji cells. The formulation does not cause hemolysis in red blood cells and is tolerant to...Continue Reading

References

Mar 19, 2002·Advanced Drug Delivery Reviews·Afsaneh LavasanifarGlen S Kwon
Mar 30, 2002·Advanced Drug Delivery Reviews·William J Egan, Georgio Lauri
Sep 3, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·D Le GarrecJ-C Leroux
Nov 18, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Geneviève GaucherJean-Christophe Leroux
May 16, 2006·Annals of the Rheumatic Diseases·A DendoovenF De Keyser
Jun 16, 2007·Journal of the American Chemical Society·Rodney P FeazellStephen J Lippard
Dec 24, 2009·Bioconjugate Chemistry·Carlos TassaStanley Y Shaw
Mar 23, 2010·Advanced Drug Delivery Reviews·Vladimir Torchilin
Jan 3, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Natalia R KuznetsovaChristian Grandfils

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Citations

Jun 15, 2016·Chemical Reviews·Jing M RenGreg G Qiao
Sep 14, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Nazar Ileri ErcanRoland Faller
Mar 28, 2017·Bioconjugate Chemistry·Chelladurai Karthikeyan BalavigneswaranNira Misra
Sep 22, 2018·Macromolecular Rapid Communications·Xiaofeng GuoSan H Thang
Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Moupiya GhoshS K Pradhan
Jul 19, 2021·Advances in Colloid and Interface Science·Didier Gigmes, Thomas Trimaille
Sep 12, 2016·ACS Biomaterials Science & Engineering·Kheyanath MitraBiswajit Ray
Dec 11, 2017·ACS Biomaterials Science & Engineering·Prateek SrivastavaPartha Pratim Manna
Sep 28, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·R ChaurasiaRay J Butcher

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