Methotrexate reduces inflammatory cell numbers, expression of monokines and of adhesion molecules in synovial tissue of patients with rheumatoid arthritis

British Journal of Rheumatology
R J DolhainA M Miltenburg

Abstract

Methotrexate (MTX) is one of the most widely prescribed drugs in the treatment of rheumatoid arthritis (RA). The mechanism by which MTX exerts its anti-rheumatic effect has not yet been defined. The aim of the present study was to investigate the effect of MTX treatment (7.5-15 mg/week) on synovial tissue in RA. For this purpose, synovial biopsies were taken from 11 RA patients before and 16 weeks after initiation of MTX therapy. Immunohistochemistry was performed using monoclonal antibodies (MAb) specific for CD3, CD4, CD8, CD22, CD25, CD38, CD68, MAb67, Ki67, interferon gamma (IFN-gamma), interleukin (IL)-1alpha, IL-1beta, tumour necrosis factor alpha (TNF-alpha), E-selectin, ICAM-1 and VCAM-1. All parameters for disease activity improved during the period of treatment. Immunohistochemical analysis revealed a statistically significant decrease in scores for CD3, CD8, CD38, CD68, Ki67, IL-1beta, TNF-alpha and the adhesion molecules E-selectin and VCAM-1. The observed decrease in synovial scores for inflammatory cells, monokines and adhesion molecules suggests that the anti-inflammatory effect of MTX is, in part, dependent on a reduction in monokine-inducible vascular adhesion molecules and subsequent reduction of cell traffic ...Continue Reading

Citations

Nov 16, 2011·Rheumatology International·Lucia FeketeováPavel Babál
Oct 11, 2005·Archives of Dermatological Research·Ayca Cordan YaziciBahar Tasdelen
Mar 19, 2013·Cell Biochemistry and Biophysics·Olfat G ShakerAli Ashmaui
Jun 9, 2005·Best Practice & Research. Clinical Rheumatology·Danielle Gerlag, Paul P Tak
Mar 23, 2011·Cellular & Molecular Immunology·Jing LiuBo Huang
Nov 20, 2002·British Journal of Haematology·Michael Y ShapiraAnthony Verstanding
Nov 6, 2002·Journal of Cellular and Molecular Medicine·Andra BalanescuDenisa Predeteanu
Jun 25, 2005·Annals of the Rheumatic Diseases·M D SmithUNKNOWN OMERACT synovial special interests group
May 18, 2005·Annals of the Rheumatic Diseases·J K Franz, G-R Burmester
May 26, 1999·Annals of the Rheumatic Diseases·D M ChangM L Chang
Jun 30, 2000·Annals of the Rheumatic Diseases·B BresnihanF Breedveld
Dec 19, 2003·Arthritis Research & Therapy·Barry Bresnihan
Feb 15, 2001·Arthritis Research·W B van den Berg
Jun 6, 2009·Arthritis Research & Therapy·Erik af KlintStaffan Lindblad
May 24, 2012·Arthritis Research & Therapy·Karina Roxana GheorgheMarina Korotkova
Aug 21, 2013·Clinical and Experimental Medicine·Tamilselvi ElangoSwapna Subramanian
Jun 7, 2005·Expert Opinion on Emerging Drugs·Jasper J HaringmanPaul P Tak
May 1, 2009·Arthritis and Rheumatism·John A Hamilton, Paul P Tak
Jan 11, 2005·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Andrew JohnstonHelgi Valdimarsson
May 5, 2001·Expert Opinion on Pharmacotherapy·F Sánchez-Madrid, R González-Amaro
Apr 21, 2016·BMC Musculoskeletal Disorders·Maria FilkovaJames Galloway
Feb 1, 2000·Baillière's Best Practice & Research. Clinical Rheumatology·B Bresnihan, P P Tak
Jul 21, 2016·Clinical and Experimental Medicine·Tamilselvi ElangoPushpa Gnanaraj
Nov 5, 2016·Nature Reviews. Rheumatology·Philip M BrownJohn D Isaacs
Jul 14, 2016·Pathogens and Disease·Shivaprasad H VenkateshaKamal D Moudgil

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.