Methoxychlor stimulates the mouse lactoferrin gene promoter through a GC-rich element

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
Zhiping Zhang, Christina T Teng

Abstract

The lactoferrin gene in the mouse uterus is a target gene for natural estrogens and xenoestrogens. One of the xenoestrogens is methyoxychlor, an insecticide that displays both estrogenic and antiandrogenic activities. Recently, methyoxychlor was found to stimulate lactoferrin gene expression in the uterus of an estrogen receptor null mouse. The present study is designed to uncover the methoxychlor response region in the mouse lactoferrin gene promoter. A series of different lengths of the mouse lactoferrin gene 5' flanking region were linked to a chloramphenicol acetyltransferase (CAT) reporter construct and transfected into human endometrial carcinoma HEC-1B cells, an estrogen receptor null cell line, in order to examine the methoxychlor response. The transfected cells were treated with methoxychlor or the metabolite of methoxychlor, HPTE, and the CAT reporter activities were measured. Constructs that contain a mouse lactoferrin 5' region longer than 100 bp were activated more than twofold by both methoxychlor and HPTE. The activation of the CAT reporter by the chemicals was dose dependent and reached saturation. Additional deletion mutants within the 100-bp region were tested, and a GC-rich sequence (GC-II) that we have previ...Continue Reading

References

Feb 1, 1989·Endocrinology·C T TengJ A McLachlan
Nov 1, 1994·Reproductive Toxicology·A M Cummings, J L Metcalf
Jan 1, 1994·Reproductive Toxicology·W J SwartzW D Johnson
Sep 1, 1995·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·A M Cummings, J L Metcalf
Jul 1, 1996·Reproductive Toxicology·V P EroschenkoW F Sims
Jul 1, 1997·Critical Reviews in Toxicology·A M Cummings
May 7, 1999·Environmental Health Perspectives·H R AndersenA M Soto

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Citations

Mar 12, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Christina T Teng
Jul 1, 2005·Environmental Toxicology and Pharmacology·Harmit S Ranhotra, Christina T Teng
Sep 24, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Rosemary A SchuhGary Fiskum
Jan 22, 2004·Experimental Biology and Medicine·Kah Poh TanLilian U Thompson

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