PMID: 2506016Sep 1, 1989Paper

Methyl-coenzyme-M reductase from Methanobacterium thermoautotrophicum (strain Marburg). Purity, activity and novel inhibitors

European Journal of Biochemistry
J EllermannA Berkessel

Abstract

Methyl-coenzyme-M reductase from Methanobacterium thermoautotrophicum (strain Marburg) was purified to a stage where, besides the alpha, beta and gamma subunits, no additional polypeptides were detectable in the preparation. Under appropriate conditions the enzyme was found to catalyze the reduction of methyl-CoM with 7-mercaptoheptanoylthreonine phosphate (H-S-HTP) to CH4 at a specific rate of 2.5 mumol.min-1.mg protein-1. This finding contradicts a recent report that methyl-CoM reductase is only active when some contaminating proteins are present. The two polypeptides encoded by the open reading frames ORF1 and ORF2 of the methyl-CoM reductase transcription unit did not co-purify with the alpha, beta and gamma subunits. They were neither required nor did they stimulate the activity under the assay conditions. 3-Bromopropanesulfonate (apparent Ki = 0.05 microM) and 2-azidoethanesulfonate (apparent Ki = 1 microM) were found to be two new competitive inhibitors of methyl-CoM reductase. Both inhibitors were considerably more effective than the "classical" 2-bromoethanesulfonate (apparent Ki = 4 microM).

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Aug 1, 1988·Molecular & General Genetics : MGG·A KleinE Muth
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D S CramJ N Reeve

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Citations

Sep 15, 2009·Applied and Environmental Microbiology·Thomas E Freitag, James I Prosser
Mar 28, 2013·Archaea : an International Microbiological Journal·Christoph WredeMichael Hoppert
Jul 30, 2004·Journal of Applied Microbiology·E M UngerfeldY Liu
Oct 26, 2012·Environmental Microbiology·Jana MiluckaSeigo Shima
May 4, 2016·Proceedings of the National Academy of Sciences of the United States of America·Evert C DuinMaik Kindermann
Jan 1, 1992·Critical Reviews in Biochemistry and Molecular Biology·J G Ferry
Sep 15, 2004·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Meike GoenrichRudolf K Thauer
Oct 15, 1993·European Journal of Biochemistry·L G BonackerR K Thauer
Sep 13, 2006·The Journal of Biological Chemistry·Ryan C KunzStephen W Ragsdale
Mar 17, 1999·FEMS Microbiology Reviews·J G Ferry

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