Methyl jasmonate enhances the radiation sensitivity of esophageal carcinoma cells by inhibiting the 11-ketoprostaglandin reductase activity of AKR1C3

Cancer Management and Research
Xiaoying LiShao-Qian Sun

Abstract

In our previous study, we found that AKR1C3 was a radioresistance gene in KY170R cells. Downregulating the expression of AKR1C3 could enhance the radiosensitivity of esophageal carcinoma cells. In this study, we investigated whether methyl jasmonate (MeJ), an inhibitor of Aldo-keto reductase family1 member C3 (AKR1C3), could overcome radiation resistance in AKR1C3 highly expressed cells. We used clone formation assays to detect radiosensitivity effects. Flow cytometry assays were used to detect reactive oxygen species (ROS) accumulation and apoptosis. Enzyme linked immunosorbent assays (ELISAs) were used to detect the concentrations of prostaglandin F2 (PGF2) and prostaglandin D2 (PGD2) in the cells after incubation with MeJ. Western blotting was used to detect AKR1C3 and peroxisome proliferator-activated receptor gamma (PPARγ) expression. We found that AKR1C3 was highly expressed in radioresistant esophageal carcinoma cells. MeJ inhibited the expression of AKR1C3 and enhanced the radiation sensitivity of esophageal carcinoma cells expressing high levels of AKR1C3 (P<0.05). MeJ could inhibit the 11-ketoprostaglandin reductase activity of AKR1C3 in a dose-dependent manner in KY170R cells. Incubation of KY170R cells with 200 µmol...Continue Reading

Citations

Mar 10, 2021·Journal of Cancer Research and Clinical Oncology·Hongfang ZhangShenglin Ma
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Iwona Jarocka-Karpowicz, Agnieszka Markowska
May 29, 2020·Journal of Medicinal Chemistry·Yang LiuHaopeng Sun

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Methods Mentioned

BETA
X-ray
protein assay
electrophoresis
flow cytometry
ELISA
irradiated

Software Mentioned

Statistical Package for Social Sciences ( SPSS

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