Methyl Protodioscin Induces Apoptosis in Human Osteosarcoma Cells by Caspase-Dependent and MAPK Signaling Pathways

Journal of Agricultural and Food Chemistry
Shun-Cheng TsengChiu-Liang Chen


Methyl protodioscin (MPD), a furostanol saponin derived from the rhizomes of Dioscorea collettii var. hypoglauca (Dioscoreaceae), has been shown to exhibit broad bioactivities such as anti-inflammation and antitumor activities. Here, we explored the molecular mechanisms by which MPD induced apoptosis in MG-63 cells. The data showed that MPD significantly suppressed cell growth (cell viabilities: 22.5 ± 1.9% for 8 μM MPD versus 100 ± 1.4% for control, P < 0.01) and enhanced cell apoptosis. The exposure to MPD resulted in a significant induction of reactive oxygen species, loss of mitochondrial membrane potential, and activation of caspase-9 and caspase-3 (P < 0.01, all cases). Furthermore, treatment with MPD increased the levels of phosphorylated JNK and p38 MAPK and markedly decreased the levels of phosphorylated ERK in MG-63 cells. Co-administration of the JNK-specific antagonist, the p38-specific antagonist, or the caspase antagonist (P < 0.05, all cases) has reversed the apoptotic effects in MPD treatment. We also found that exposure to MPD resulted in a significant reduction in the protein level of anti-apoptotic proteins Bcl-2, survivin, and XIAP (P < 0.05, all cases). In conclusion, our results indicate that MPD induces a...Continue Reading


Apr 26, 2003·The Journal of Organic Chemistry·Mao S ChengZhen Yang
Aug 7, 2003·Cancer Investigation·Ke Hu, Xinsheng Yao
Oct 16, 2003·Journal of Agricultural and Food Chemistry·Deng-Jye YangLucy Sun Hwang
Jun 22, 2010·Apoptosis : an International Journal on Programmed Cell Death·Joanna SkommerSubhadip Raychaudhuri
Apr 10, 2012·Apoptosis : an International Journal on Programmed Cell Death·Yao YaoYu-Xin Li
Aug 21, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Zhengqi ChangXiuchun Yu
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Mar 14, 2014·The Journal of Pharmacology and Experimental Therapeutics·Jin Wang, Wei Li
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Aug 10, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xin PengQingyong Ji
Jun 22, 2016·Apoptosis : an International Journal on Programmed Cell Death·Zhi-Feng LiuLei Su

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