Methyl tert-butyl ether (MTBE) induced Ca(2+)-dependent cytotoxicity in isolated rabbit tracheal epithelial cells

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Yajing WangXiaodong Han

Abstract

As a volatile synthetic organic chemical, methyl tert-butyl ether (MTBE) was the most common gasoline additive. The increasing use of MTBE raised concern over its health safety. Inhalation was the principle route of exposure for the general population. This study used a model of rabbit tracheal epithelial cells (RTEs) in primary culture to investigate the cytotoxic effects induced by MTBE and the potential mechanism. RTEs were incubated with medium alone (control), 0.5, 50, 5000ppm MTBE respectively. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazo liumbromide) assay, staining with fluorescein diacetate, propidium iodide and lactate dehydrogenase leakage ratio were used to assess MTBE cytotoxicity on cells. We also observed a significant elevation in cytosolic Ca2+ by fluorescence probe Fluo-3AM at 3, 6 and 12h following exposure to MTBE. Loss of mitochondrial membrane potential (MMP) was detected following 12 and 24h treatment of NP and assessment by rhodamine 123 (Rh123) staining. Activity changes of the Ca(2+)-ATPase, Ca(2+)-Mg(2+)-ATPase following MTBE treatment displayed a similar trend, suggesting an initial elevation before 6h and subsequent dramatic decrease at 12h. Our results demonstrated that induction of cell i...Continue Reading

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Citations

Mar 16, 2013·In Vitro Cellular & Developmental Biology. Animal·Hong-Can ShiYan-Jun Zeng
Dec 19, 2015·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Arastoo SaeediAfshin Mohammadi-Bardbori
Jun 20, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Haitao ShenXianghong Zhang
Sep 1, 2019·Journal of Environmental Sciences (China)·Yue TangYuguo Du

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