Abstract
We propose to further research the protective effect of MMI on myocardium ischemic rat model and H9c2 cells that underwent cell apoptosis induced by hypoxia. We established the myocardium ischemic rat model via the cardiac surgical procedures in vivo and treated the model rats with different concentration of MMI. In vitro, with the pretreatment of MMI for 12 h in the model of Na2S2O4-induced hypoxia injury, the H9c2 cells viability was determined by MTT assay. We found that MMI had significantly improved cardiac function of the myocardial ischemia, and significantly decreased the reactive oxygen species level. The expression of P53, Bcl-2, Bax, and caspase-9 was also induced by MMI. In vitro study revealed a concentration-dependent increase in cell viability associated with MMI pretreatment. Annexin V-FITC and PI staining results showed that MMI had a preventive effect on hypoxia-induced apoptosis in H9c2 cells. MMI also inhibited the mitochondrial membrane potential decrease and increased total ATPase activity during hypoxia in H9c2 cells. In conclusion, MMI can enhance the cardiac function in myocardial ischemic rat and increase cell viability and attenuate the apoptosis in H9c2 cells induced by hypoxia, which was associated ...Continue Reading
References
Feb 14, 1998·Phytochemistry·W M Keung, B L Vallee
Dec 10, 1999·British Journal of Pharmacology·B DeodatoF Squadrito
Feb 9, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Zuo-Hui ShaoChun-Su Yuan
Jul 10, 2003·Menopause : the Journal of the North American Menopause Society·Jean WooAprille Sham
Nov 25, 2003·The Journal of Biological Chemistry·Joslyn K BrunelleNavdeep S Chandel
Dec 4, 2003·Journal of Pharmacological Sciences·Yeo-Ok HanDong-Hyun Kim
Mar 31, 2004·The Journal of Pharmacology and Experimental Therapeutics·Jing LiuTak Ming Wong
Mar 4, 2005·Biological & Pharmaceutical Bulletin·Hae-Ung LeeDong-Hyun Kim
Apr 12, 2005·Journal of Pharmacological Sciences·Hae-Ung LeeDong-Hyun Kim
Dec 30, 2006·The Journal of Pharmacology and Experimental Therapeutics·Jin-Sun ParkHee-Sun Kim
Sep 18, 2007·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·L WuL Li
Nov 6, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Courtney L WilliamsonJohn M Hollander
Nov 13, 2007·Bioorganic & Medicinal Chemistry·Kyoung Ah KangJin Won Hyun
Jun 4, 2013·Gene·Hua-Sheng DingQin-Qin Yu
Dec 6, 2014·Life Sciences·Dongsheng YuJing Shang
Sep 15, 2015·Life Sciences·Chun-Ling ChenJiang Wang
Nov 18, 2015·Pathology, Research and Practice·Xiaojuan LiuJiahai Shi