Methylammonium-resistant mutants of Nicotiana plumbaginifolia are affected in nitrate transport

Molecular & General Genetics : MGG
C GodonM Caboche

Abstract

This work reports the isolation and preliminary characterization of Nicotiana plumbaginifolia mutants resistant to methylammonium. Nicotiana plumbaginifolia plants cannot grow on low levels of nitrate in the presence of methylammonium. Methylammonium is not used as a nitrogen source, although it can be efficiently taken up by Nicotiana plumbaginifolia cells and converted into methylglutamine, an analog of glutamine. Glutamine is known to repress the expression of the enzymes that mediate the first two steps in the nitrate assimilatory pathway, nitrate reductase (NR) and nitrite reductase (NiR). Methylammonium has therefore been used, in combination with low concentrations of nitrate, as a selective agent in order to screen for mutants in which the nitrate pathway is de-repressed. Eleven semi-dominant mutants, all belonging to the same complementation group, were identified. The mutant showing the highest resistance to methylammonium was not affected either in the utilization of ammonium, accumulation of methylammonium or in glutamine synthase activity. A series of experiments showed that utilization of nitrite by the wild-type and the mutant was comparable, in the presence or the absence of methylammonium, thus suggesting that ...Continue Reading

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Citations

Apr 5, 2000·Biochimica Et Biophysica Acta·B G Forde
Mar 6, 1999·Current Opinion in Plant Biology·F Daniel-VedeleM Caboche
Oct 20, 2005·Journal of Bioscience and Bioengineering·Takehiko ShiraishiAkio Kobayashi
Jun 19, 2007·The Journal of Biological Chemistry·Judith WirthAlain Gojon

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