Methylation and Gene Expression of BCAT1 and IKZF1 in Colorectal Cancer Tissues

Clinical Medicine Insights. Oncology
Maher JediErin L Symonds

Abstract

The genes BCAT1 and IKZF1 are hypermethylated in colorectal cancer (CRC), but little is known about how this relates to gene expression. This study assessed the relationship between methylation and gene expression of BCAT1 and IKZF1 in CRC and adjacent non-neoplastic tissues. The tissues were obtained at surgery from 36 patients diagnosed with different stages of CRC (stage I n = 8, stage II n = 13, stage III n = 10, stage IV n = 5). Methylated BCAT1 and IKZF1 were detected in 92% and 72% CRC tissues, respectively, with levels independent of stage (P > .05). In contrast, only 31% and 3% of non-neoplastic tissues were methylated for BCAT1 and IKZF1, respectively (P < .001). The IKZF1 messenger RNA (mRNA) expression was significantly lower in the cancer tissues compared with that of non-neoplastic tissues, whereas the BCAT1 mRNA levels were similar. The latter may be due to the BCAT1 polymerase chain reaction assay detecting more than 1 mRNA transcript. Further studies are warranted to establish the role of the epigenetic silencing of IKZF1 in colorectal oncogenesis.

References

Jan 27, 2000·Cell·D Hanahan, R A Weinberg
Mar 27, 2002·Nature Reviews. Immunology·Katia Georgopoulos
Aug 3, 2002·Oncogene·Melanie Ehrlich
Jul 23, 2003·Bioinformatics·Perikles Simon
Mar 6, 2004·Cancer Metastasis Reviews·Yutaka Kondo, Jean-Pierre J Issa
Oct 7, 2004·Journal of the National Cancer Institute·Jessica B O'ConnellClifford Y Ko
Jun 3, 2005·The Journal of Nutrition·Robert A HarrisMariko Obayashi
Sep 29, 2006·World Journal of Gastroenterology : WJG·Reigetsu YoshikawaTomoko Hashimoto-Tamaoki
Sep 26, 2007·PLoS Genetics·Kornel E SchuebelStephen B Baylin
Mar 14, 2008·The New England Journal of Medicine·Manel Esteller
Feb 6, 2010·Cancer Metastasis Reviews·Myoung Sook KimDavid Sidransky
Nov 26, 2010·Annual Review of Pathology·Eric R Fearon
Jul 9, 2011·Molecular Cancer Research : MCR·Biola M JavierreEsteban Ballestar
Jul 12, 2011·Epigenetics : Official Journal of the DNA Methylation Society·Fang Fang ZhangAlfredo Morabia
Jul 20, 2012·Nature·UNKNOWN Cancer Genome Atlas Network
Apr 13, 2013·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Zhenfa ZhangZhe Liu
Apr 17, 2013·The Journal of Cell Biology·Begoña DíazSara A Courtneidge
Feb 4, 2014·BMC Cancer·Susan M MitchellPeter L Molloy
Jan 27, 2015·International Journal of Molecular Sciences·Jennifer Mun-Kar Ng, Jun Yu
Feb 1, 2015·BMC Biology·Marc Jung, Gerd P Pfeifer
May 1, 2015·PloS One·Susanne K PedersenLawrence C LaPointe
May 30, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·John B KisielDavid A Ahlquist
Jan 15, 2016·Clinical and Translational Gastroenterology·Erin L SymondsGraeme P Young
Feb 5, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Angel Diaz-LagaresJuan Sandoval
Dec 7, 2017·Current Opinion in Clinical Nutrition and Metabolic Care·Elitsa A Ananieva, Adam C Wilkinson

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Citations

Oct 19, 2019·World Journal of Clinical Cases·Yu-Xia Zhan, Guang-Hua Luo
Dec 6, 2019·Frontiers in Genetics·Warwick J LockeJason P Ross
Sep 23, 2020·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Benjamin L MusherGraeme P Young

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Methods Mentioned

BETA
surgical resection
PCR
biopsy

Software Mentioned

GraphPad Prism
GraphPad
Gene application

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