PMID: 32509187Jun 9, 2020Paper

Methylation and serum response factor mediated in the regulation of gene ARRDC3 in breast cancer

American Journal of Translational Research
Sheyu LinShuaiyao Wang

Abstract

Breast cancer poses a serious threat to women's life and health and many factors contribute to breast cancer including gene mutation and epigenetics. Gene ARRDC3 was usually repressed in breast cancer and methylation in promoter was reported to be involved in gene ARRDC3 expression regulation. To this end, the methylation status for gene ARRDC3 promoter was assayed by the Massarray quantitative method. The results indicated that different methylation level CpG sites including CpG_6, CpG_13.14, CpG_17.18, and CpG_25 existed between the tumor tissue and the adjacent normal tissue. In order to further verify whether methylation participated in gene ARRDC3 expression, three cell lines were treated with methylation inhibitor Aza-2'-deoxycytidine including A-375, HepG2, and MDA-MB-231. The results revealed that methylation inhibition observably increased ARRDC3 mRNA expression. Then we confirmed the effective length of promoter through the fluorescence report assay used for further analysis. The results showed that the 1746 bp length promoter produced the maximum fluorescence signal. To obtain the direct evidence that methylation in gene ARRDC3 promoter mediated in ARRDC3 expression regulation, the promoter plasmid was methylated by ...Continue Reading

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