Methylation changes in porcine primordial germ cells

Molecular Reproduction and Development
Stoyan G PetkovGary B Anderson

Abstract

Epigenetic re-programming is an important event in the development of primordial germ cells (PGC) into functional gametes, characterized by genome-wide erasure of DNA methylation and re-establishment of epigenetic marks, a process essential for restoration of the potential for totipotency. In this study changes in the methylation status of centromeric repeats and two IGF2-H19 differentially methylated domain (DMD) sequences were examined in porcine PGC between Days 24 and 31 of pregnancy. The methylation levels of centromeric repeats and IGF2-H19 DMD sequences decreased rapidly from Days 24 to 28 in both male and female PGC. At Days 30 and 31 of pregnancy centromeric repeats and IGF2-H19 DMD sequences acquired new methylation in male PGC, while in female PGC these sequences were completely demethylated by Day 30 and remained hypomethylated at Day 31. To characterize methylation changes that PGC undergo in culture, the methylation status of embryonic germ cells (EGCs) derived from PGC at Day 26 of pregnancy was examined. Centromeric repeats and IGF2-H19 DMD sequences were similarly methylated in both male and female EGC and hypermethylated in female EGC compared with female PGC at the same embryonic age. Our results show that, s...Continue Reading

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Citations

Dec 25, 2012·Stem Cells and Development·Bong-Wook ParkJulang Li
Mar 1, 2011·BMC Developmental Biology·Sara M W HyldigRamiro Alberio
Sep 24, 2013·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Yimei CongZhonghua Liu
Nov 29, 2015·Cell and Tissue Research·Yu ZhangZhonghua Liu
Mar 30, 2010·Reproduction in Domestic Animals = Zuchthygiene·J WenZ Li
Aug 13, 2009·Reproduction in Domestic Animals = Zuchthygiene·O OestrupP Hyttel
Aug 1, 2012·Reproduction in Domestic Animals = Zuchthygiene·R Alberio, A R Perez
Jun 7, 2016·Scientific Reports·Hanning WangJianyong Han
Sep 12, 2018·Cellular Reprogramming·Kwang-Hwan ChoiChang-Kyu Lee
Mar 4, 2011·Reproduction, Fertility, and Development·Hélène JammesPascale Chavatte-Palmer
May 13, 2021·Stem Cell Reports·Ramiro AlberioM Azim Surani
Apr 3, 2012·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Zengxiang PanHonglin Liu

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