Methylation-free site patterns along a 1-Mb locus on Chr19 in cancerous and normal cells are similar. A new fast approach for analyzing unmethylated CCGG sites distribution

Molecular Genetics and Genomics : MGG
T L AzhikinaEugene D Sverdlov

Abstract

We describe a newly developed technique for rapid identification of positions of genomic DNA breaks, preexisting or introduced by specific digestion, in particular, by restriction endonucleases (RIDGES). We applied RIDGES in analyzing unmethylated CCGG sites distribution along a 1-Mb long genome region (D19S208-COX7A1 on chromosome 19) in cancerous and normal lung tissues. Both tissues were characterized by a profoundly uneven density of unmethylated sites along the fragment. Interestingly, the distribution of hypomethylated regions did not correlate with gene locations within the fragment, and one of the most hypomethylated areas contained practically no genes. We also demonstrated that the methylation pattern of a long genome DNA fragment was rather stable and practically unchanged in human lung cancer tissue as compared with its normal counterpart, in accordance with the suggestion (Ross et al. in Nat Genet 24:227-235, 2000) that cell lines of common origin have typically similar transcription profiles. An analogous suggestion might probably be made for global methylation patterns of genomic DNA.

References

Oct 20, 1995·Science·V E VelculescuK W Kinzler
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Mar 24, 2000·Trends in Genetics : TIG·S B Baylin, J G Herman
Mar 10, 2001·Nature·E S LanderUNKNOWN International Human Genome Sequencing Consortium
Dec 1, 2001·Biochemical and Biophysical Research Communications·C KusuiY Murata
Feb 28, 2002·Nucleic Acids Research·Péter AdorjánAlexander Olek
May 22, 2002·Nature Genetics·Bernard W FutscherFrederick E Domann
Aug 3, 2002·Oncogene·Melanie Ehrlich
Sep 20, 2002·BioTechniques·Mario F Fraga, Manel Esteller
Sep 25, 2003·Cellular and Molecular Life Sciences : CMLS·F Antequera
Oct 31, 2003·Trends in Genetics : TIG·Yuk Fai Leung, Duccio Cavalieri
Nov 9, 2004·Current Opinion in Genetics & Development·Suyinn Chong, Emma Whitelaw

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Citations

Jul 17, 2007·Biochemistry. Biokhimii︠a︡·S B AkopovL G Nikolaev
Jan 22, 2008·Biochemistry. Biokhimii︠a︡·I V GainetdinovE D Sverdlov
Oct 13, 2009·Biochemistry. Biokhimii︠a︡·Y V SkvortsovaE D Sverdlov
May 25, 2010·Epigenomics·Melanie Ehrlich
Apr 11, 2014·BioMed Research International·Oksana S BychenkoEugene D Sverdlov
Feb 23, 2012·Gene·Konstantin BaskaevAnton A Buzdin

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