Methylation of 23S rRNA nucleotide G745 is a secondary function of the RlmAI methyltransferase

RNA
Mingfu LiuStephen Douthwaite

Abstract

Several groups of Gram-negative bacteria possess an RlmA(I) methyltransferase that methylates 23S rRNA nucleotide G745 at the N1 position. Inactivation of rlmA(I) in Acinetobacter calcoaceticus and Escherichia coli reduces growth rates by at least 30%, supposedly due to ribosome malfunction. Wild-type phenotypes are restored by introduction of plasmid-encoded rlmA(I), but not by the orthologous Gram-positive gene rlmA(II) that methylates the neighboring nucleotide G748. Nucleotide G745 interacts with A752 in a manner that does not involve the guanine N1 position. When a cytosine is substituted at A752, a Watson-Crick G745-C752 pair is formed occluding the guanine N1 and greatly reducing RlmA(I) methylation. Methylation is completely abolished by substitution of the G745 base. Intriguingly, the absence of methylation in E. coli rRNA mutant strains causes no reduction in growth rate. Furthermore, the slow-growing rlmA(I) knockout strains of Acinetobacter and E. coli revert to the wild-type growth phenotype after serial passages on agar plates. All the cells tested were pseudorevertants, and none of them had recovered G745 methylation. Analyses of the pseudorevertants failed to reveal second-site mutations in the ribosomal compone...Continue Reading

References

May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B C PerssonG R Björk
Jan 1, 1989·Annual Review of Microbiology·E Cundliffe
Jun 11, 1984·Nucleic Acids Research·C D SigmundE A Morgan
Mar 1, 1995·Antimicrobial Agents and Chemotherapy·B Weisblum
Feb 1, 1993·Journal of General Microbiology·R PalmenK J Hellingwerf
Dec 10, 1998·Nucleic Acids Research·J RozenskiJ A McCloskey
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·T AsaiC L Squires
Apr 3, 2001·Science·M M YusupovH F Noller
Aug 15, 2001·Journal of Molecular Biology·L H HansenS Douthwaite
Mar 15, 2002·Cell·Tanel Tenson, Måns Ehrenberg
Mar 15, 2002·Cell·Hitoshi Nakatogawa, Koreaki Ito
Mar 21, 2002·FEBS Letters·James Ofengand
May 23, 2002·Antimicrobial Agents and Chemotherapy·Mingfu Liu, Stephen Douthwaite
Aug 2, 2002·Molecular Cell·Jeffrey L HansenThomas A Steitz
Nov 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mingfu Liu, Stephen Douthwaite
Nov 15, 2002·The Journal of Biological Chemistry·Wayne A Decatur, Maurille J Fournier
Mar 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kalyan DasGaetano T Montelione
Mar 30, 2004·Journal of Molecular Biology·Stephen DouthwaiteJacob Poehlsgaard

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Citations

Apr 11, 2009·The Journal of Biological Chemistry·Audrey PerreaultFrancois Bachand
Jun 15, 2007·Critical Reviews in Biochemistry and Molecular Biology·Daniel N Wilson, Knud H Nierhaus
Dec 17, 2014·Biochimie·O V SergeevaP V Sergiev
May 30, 2006·The Journal of Biological Chemistry·Jonas Mengel-JørgensenFinn Kirpekar

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