Methylation of CpG islands of p16 associated with progression of primary gastric carcinomas

Laboratory Investigation; a Journal of Technical Methods and Pathology
Daya LuoDajun Deng

Abstract

Inactivation of p16 by methylation of CpG islands is a frequent early event in gastric carcinogenesis. The positive relationship between p16 methylation and the clinical characteristics of gastric carcinomas (GC) has not been reported to date. In the present study, a DHPLC assay to quantify p16 methylation was established (detection limit by fluorescence detector: 1:255 (Methlyated vs Unmethylated)). The proportion of methylated p16 in the representative samples was confirmed and standardized by clone sequencing. Then, the DHPLC and two regular methylation-specific PCR (MSP) assays were used to detect p16 methylation in 82 paired, resected GCs and their adjacent normal tissues. Results showed that the average proportion of methylated p16 in GCs was significantly higher than that in their adjacent samples (12.90 vs 0.63%; t-test P=0.005). A much higher proportion of methylated p16 was detected in GCs with metastases (local or distant) than without metastases (14.76 vs 2.61%; t-test P=0.014). A proportional relationship was observed between clinical stages and positive rates of p16 methylation in GCs and/or adjacent tissues: 27.3, 37.5, and 58.8% (by DHPLC) for stage-I, -II, -III-IV of GCs, respectively (two-sided Fisher's exact ...Continue Reading

References

Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·J G HermanS B Baylin
Oct 1, 1996·Japanese Journal of Cancer Research : Gann·M S WuJ T Lin
Jan 1, 1997·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·A S TakaokaK Imai
Jan 23, 1999·Pathology, Research and Practice·T GüntherA Roessner
Feb 13, 1999·Nature Genetics·P A Jones, P W Laird
Sep 25, 1999·International Journal of Cancer. Journal International Du Cancer·H SuzukiK Imai
Mar 29, 2000·Nucleic Acids Research·C A EadsP W Laird
Jun 1, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·Y H ShimJ Y Ro
Jan 30, 2002·Journal of Surgical Oncology·Calvin S H NgAnthony P C Yim
Jun 4, 2002·Nature Reviews. Genetics·Peter A Jones, Stephen B Baylin
Jan 23, 2003·Carcinogenesis·Dajun DengSteven M Powel
Nov 25, 2003·The New England Journal of Medicine·James G Herman, Stephen B Baylin
Aug 7, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yu SunJi-You Li
Sep 2, 2005·Nature Reviews. Genetics·Keith D Robertson

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Citations

Mar 12, 2010·World Journal of Gastroenterology : WJG·Xian-Zi WenDa-Jun Deng
Nov 24, 2017·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Baozhen ZhangDajun Deng
Nov 29, 2007·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Kirsten GrønbaekPeter A Jones
Sep 30, 2008·International Journal of Cancer. Journal International Du Cancer·Cai-Xuan DongWei-Cheng You
May 13, 2010·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·S Mohammad GanjiM Negrini
Feb 11, 2011·Journal of Surgical Oncology·Dorra Ben Ayed-GuerfaliRaja Mokdad-Gargouri
Mar 19, 2013·Journal of the Chinese Medical Association : JCMA·Peng WangBaoguo Liu
Mar 13, 2014·Molecular Biology Reports·He-Ling WangYu Zhang
Dec 4, 2019·Epigenetics : Official Journal of the DNA Methylation Society·Paiyun LiDajun Deng
Nov 26, 2011·Archives of Gynecology and Obstetrics·Lu-Lu SunKeng Shen
Aug 13, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jie CaoDajun Deng
Jul 11, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Zhaojun LiuDajun Deng
Feb 23, 2010·Molecular Cancer·Xiao-Wei ZhangWei-Jian Guo

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